Human Immunodeficiency Virus HIV CD3+ T Cells

About Human Immunodeficiency Virus HIV CD3+ T Cells

HIV CD3+ T Cells for Virology and Immune Dysfunction Research

HIV CD3+ T Cells are isolated from peripheral blood of IRB-consented adult donors diagnosed with Human Immunodeficiency Virus (HIV). These CD3+ T lymphocytes carry the hallmarks of chronic HIV infection and are valuable for investigating T cell phenotypes, exhaustion, cytokine secretion, and immune reconstitution.

What HIV Does to the T Cell Compartment

HIV is an infectious cause of acquired immunodeficiency, and the T cell compartment is where that plays out. CD4+ helper cells are the primary target of infection and are progressively depleted, while the CD8+ compartment expands in response — producing the inverted CD4/CD8 ratio that characterises untreated infection. A pan-CD3+ isolate captures both arms together, which is what you want when the ratio itself, or the interaction between the two, is the object of study.

Chronic antigen exposure also drives a distinctive exhaustion phenotype — elevated PD-1, TIM-3, LAG-3 and TIGIT with reduced proliferative and cytotoxic capacity — alongside persistent immune activation and inflammation that continues even in donors virally suppressed on therapy. That residual activation is a major current research question, and it needs cells from treated donors rather than from healthy controls.

Donor Stratification Available

• CD4+ count and CD4/CD8 ratio where recorded
• Viral load — HIV-1 RNA copies/mL where tested
• Treatment status — treatment-naive, or on suppressive ART with duration
• Immunological non-responders — donors with poor CD4 recovery despite viral suppression
• Elite controllers — available as custom collections
• HLA type and CMV serostatus on request
• Matched seronegative controls available

Product Features

• Research Use Only (RUO), CD3+ enriched T cells
• Isolated from clinically confirmed HIV donors
• High viability and CD3+ cell enrichment
• Cryopreserved in CryoStor® CS10 media
• Processed within 24 hours of collection
• Flow cytometry purity and viability data per lot
• IRB-approved collection and HIPAA-compliant handling
• Biosafety-compliant handling and shipping

De-identified Donor Data

• Confirmed HIV diagnosis
• Demographics: age, sex assigned at birth, race/ethnicity
• Clinical history and medication records available

Applications

• T cell subset analysis and immune profiling in HIV
• CD4/CD8 ratio inversion and immune reconstitution studies
• T cell exhaustion phenotyping — PD-1, TIM-3, LAG-3, TIGIT
• Chronic immune activation and residual inflammation on ART
• Viral persistence and latent reservoir research
• Cytokine production and functional capacity assays
• Target validation and preclinical drug development
• Cell therapy and immune modulation modeling

Other HIV Specimen Types

Matched material is available as HIV CD4+ T cells, CD8+ T cells, CD19+ B cells, CD56+ NK cells, PBMC, and leukopak for high-yield reservoir work.

Compliance and Quality Assurance

• IRB-approved protocols and 21 CFR Part 11–compliant e-consent
• Standardized isolation and cryopreservation
• HIPAA-compliant data protection and donor confidentiality

Ordering & Customization

HIV CD3+ T Cells are available in standard or custom aliquot sizes. For pricing, international shipments, or documentation, contact learnmore@sanguinebio.com. For the full range, see our human CD3+ T cells collection.