Human Immunodeficiency Virus HIV CD19+ B Cells

About Human Immunodeficiency Virus HIV CD19+ B Cells

HIV CD19+ B Cells for Viral Immunology and Antibody Discovery

HIV CD19+ B Cells are purified from peripheral blood of IRB-consented adult donors diagnosed with Human Immunodeficiency Virus (HIV). These B lymphocytes carry the characteristic B cell abnormalities of chronic HIV infection and support studies of humoral immune dysfunction, vaccine responsiveness, and antibody discovery. Cryopreserved for optimal viability.

What HIV Does to the B Cell Compartment

Although HIV infects CD4+ T cells rather than B cells directly, the B cell compartment is substantially disturbed — and in ways that make donor material genuinely valuable rather than merely disease-labelled:

• Polyclonal hypergammaglobulinemia — elevated total immunoglobulin from chronic activation, alongside paradoxically poor antigen-specific responses
• Exhausted “tissue-like memory” B cells — a CD21-low, inhibitory-receptor-high population expanded in viremic donors
• Loss of resting memory B cells, which underlies impaired responses to vaccination even in donors on suppressive therapy
• Increased apoptosis and altered BCR signalling
• Broadly neutralizing antibody (bNAb) development — a subset of donors generates bNAbs after years of infection, and their B cells are the source material for isolating and characterising those lineages

That last point is why HIV donor B cells matter well beyond HIV itself: bNAb lineage tracing from elite neutralisers underpins much of current HIV vaccine immunogen design.

Donor Stratification Available

• Viral load and CD4+ count where tested
• Treatment status — viremic, or suppressed on ART with duration
• Elite neutralisers — donors with broad serum neutralising activity, available as custom collections
• Duration of infection, which correlates with bNAb maturation
• Matched seronegative controls available

Product Features

• Research Use Only (RUO), CD19+ B cells
• Isolated from HIV+ donors with confirmed clinical diagnosis
• High purity and viability
• Cryopreserved using CryoStor® CS10 media
• Processed within 24 hours of collection
• Flow cytometry purity and viability data per lot
• IRB-approved protocols and HIPAA-compliant handling
• Biosafety-compliant handling and shipping

De-identified Donor Data

• Confirmed diagnosis of HIV
• Demographic data: age, sex assigned at birth, race/ethnicity
• Medication and clinical history available

Applications

• B cell subset profiling and immune phenotyping in HIV
• Exhausted and tissue-like memory B cell characterisation
• Broadly neutralizing antibody isolation and lineage tracing
• BCR repertoire sequencing
• Vaccine responsiveness and immunogen design research
• BCR signalling and B cell function studies
• Biomarker discovery and therapeutic target validation

Other HIV Specimen Types

Matched material is available as HIV CD3+ T cells, CD4+ T cells, CD8+ T cells, CD56+ NK cells, PBMC, and leukopak.

Compliance and Quality Assurance

• IRB-approved collections with 21 CFR Part 11–compliant e-consent
• Standardized processing and cryopreservation protocols
• HIPAA-compliant donor data protection

Ordering & Customization

HIV CD19+ B Cells are available in standard or custom aliquot sizes. For pricing, international shipping, documentation, or regulatory support, please contact learnmore@sanguinebio.com. For the full range, see our human CD19+ B cells collection.