Healthy CD19+ B Cells

Healthy CD19+ B Cells for Immunology and Antibody Discovery Research

Healthy CD19 B Cells are purified from the peripheral blood of healthy, IRB-consented, clinically screened adult donors and cryopreserved within 24 hours of collection. B lymphocytes are the effectors of humoral immunity, and these primary human cells serve as the baseline reference material for studying antibody production, B cell receptor (BCR) signalling, and immune regulation. Disease-state CD19+ B cells are also available for comparative work.

B Cell Biology and Isolation

• CD19 is the canonical pan-B-cell marker, expressed from the pro-B stage through to activated B cells and most plasmablasts, and lost on fully differentiated plasma cells. It is also the most clinically established target antigen in B-cell-directed cell therapy.
• Cells are isolated from apheresis or peripheral blood mononuclear cell starting material by immunomagnetic selection. Negative selection leaves the B cell surface untouched and is preferred where downstream receptor engagement or activation state matters; positive selection is available where maximum purity is the priority.
• The isolated fraction contains the physiological spread of circulating B cell subsets, distinguishable by CD27, IgD, CD24, and CD38 expression: transitional and naive B cells, unswitched and class-switched memory B cells, and a small plasmablast population.
• CD19 is physically and functionally coupled to the BCR complex, where it acts with CD21 and CD81 as a co-receptor that lowers the threshold for antigen-driven activation – making these cells directly suited to signalling studies.
• Primary human B cells are short-lived in culture without support, so activation systems supplying CD40 ligand, IL-4, IL-21, or TLR9 stimulation are typically used for proliferation, class switching, and differentiation assays.
• Cryopreservation in CryoStor CS10 with processing inside 24 hours of collection preserves viability and function for thaw-and-use assay formats.

Donor Selection and Characterisation

• Age band and sex assigned at birth
• Race and ethnicity
• CMV and EBV serostatus, which is relevant to memory B cell composition and to viral antigen-specific work
• HLA type where typing is available, for use in cell therapy target-cell and allogeneic response studies
• ABO and Rh blood type
• Body mass index, medication and allergy history, and repeat-donor availability for longitudinal or paired-lot studies

Product Features

• Research Use Only (RUO), CD19+ B cells
• Isolated from healthy, clinically screened donors
• High purity and viability (≥90% CD19+ cells)
• Cryopreserved using CryoStor® CS10 media
• Processed within 24 hours of collection
• Negative or positive selection available on request
• Standard or custom aliquot sizes
• IRB-approved protocols and HIPAA-compliant handling

De-identified Donor Data

• Comprehensive donor screening and verified health status
• Demographic data: age, sex assigned at birth, race/ethnicity
• Medication and allergy history available
• Infectious disease testing results, including CMV and EBV serology
• HLA type and blood type where available

Applications

• B cell activation, proliferation, and differentiation assays using CD40L, IL-4, IL-21, or TLR agonist stimulation
• Antibody production and class-switch recombination studies
• B cell receptor signalling analysis, including phosphoflow and kinase inhibitor profiling
• Antibody discovery workflows, including single B cell cloning and BCR repertoire sequencing
• CAR-T and CAR-NK development as CD19+ target cells for cytotoxicity, killing, and potency assays
• Immunophenotyping panel design, optimisation, and instrument standardisation
• Drug screening and mechanism-of-action work for B-cell-depleting antibodies, bispecific engagers, and BTK inhibitors
• Baseline reference material for comparison against disease-state B cell products

Other Healthy Immune Cell Subsets

• Healthy CD3+ T Cells
• Healthy CD4+ T Cells
• Healthy CD8+ T Cells
• Healthy CD56+ NK Cells

Compliance and Quality Assurance

• IRB-approved collections with 21 CFR Part 11-compliant e-consent
• Standardized processing and cryopreservation protocols
• HIPAA-compliant donor data protection

Ordering & Customization

Healthy CD19 B Cells are available in standard or custom aliquot sizes. For pricing, international shipping, documentation, or regulatory support, please contact learnmore@sanguinebio.com. To browse the full range of isolated B cell products and current availability, visit our human CD19+ B cells page.