Healthy CD19+ B Cells
Powered by BiozAbout:Healthy CD19 B Cells are isolated from the peripheral blood of consented healthy donors, cryopreserved in CryoStor CS10 at ≥90% CD19+ purity. Ideal for B cell activation and differentiation assays, BCR signalling, antibody discovery, CD19+ target cells for CAR-T and CAR-NK work, and immunophenotyping panel development. Donor selection by age, sex, ethnicity, CMV/EBV serostatus, HLA, and blood type. Disease-state CD19+ B cells are also available.
About Healthy CD19+ B Cells
Healthy CD19+ B Cells for Immunology and Antibody Discovery Research
Healthy CD19 B Cells are purified from the peripheral blood of healthy, IRB-consented, clinically screened adult donors and cryopreserved within 24 hours of collection. B lymphocytes are the effectors of humoral immunity, and these primary human cells serve as the baseline reference material for studying antibody production, B cell receptor (BCR) signalling, and immune regulation. Disease-state CD19+ B cells are also available for comparative work.
B Cell Biology and Isolation
- CD19 is the canonical pan-B-cell marker, expressed from the pro-B stage through to activated B cells and most plasmablasts, and lost on fully differentiated plasma cells. It is also the most clinically established target antigen in B-cell-directed cell therapy.
- Cells are isolated from apheresis or peripheral blood mononuclear cell starting material by immunomagnetic selection. Negative selection leaves the B cell surface untouched and is preferred where downstream receptor engagement or activation state matters; positive selection is available where maximum purity is the priority.
- The isolated fraction contains the physiological spread of circulating B cell subsets, distinguishable by CD27, IgD, CD24, and CD38 expression: transitional and naive B cells, unswitched and class-switched memory B cells, and a small plasmablast population.
- CD19 is physically and functionally coupled to the BCR complex, where it acts with CD21 and CD81 as a co-receptor that lowers the threshold for antigen-driven activation – making these cells directly suited to signalling studies.
- Primary human B cells are short-lived in culture without support, so activation systems supplying CD40 ligand, IL-4, IL-21, or TLR9 stimulation are typically used for proliferation, class switching, and differentiation assays.
- Cryopreservation in CryoStor CS10 with processing inside 24 hours of collection preserves viability and function for thaw-and-use assay formats.
Donor Selection and Characterisation
- Age band and sex assigned at birth
- Race and ethnicity
- CMV and EBV serostatus, which is relevant to memory B cell composition and to viral antigen-specific work
- HLA type where typing is available, for use in cell therapy target-cell and allogeneic response studies
- ABO and Rh blood type
- Body mass index, medication and allergy history, and repeat-donor availability for longitudinal or paired-lot studies
Product Features
- Research Use Only (RUO), CD19+ B cells
- Isolated from healthy, clinically screened donors
- High purity and viability (≥90% CD19+ cells)
- Cryopreserved using CryoStor® CS10 media
- Processed within 24 hours of collection
- Negative or positive selection available on request
- Standard or custom aliquot sizes
- IRB-approved protocols and HIPAA-compliant handling
De-identified Donor Data
- Comprehensive donor screening and verified health status
- Demographic data: age, sex assigned at birth, race/ethnicity
- Medication and allergy history available
- Infectious disease testing results, including CMV and EBV serology
- HLA type and blood type where available
Applications
- B cell activation, proliferation, and differentiation assays using CD40L, IL-4, IL-21, or TLR agonist stimulation
- Antibody production and class-switch recombination studies
- B cell receptor signalling analysis, including phosphoflow and kinase inhibitor profiling
- Antibody discovery workflows, including single B cell cloning and BCR repertoire sequencing
- CAR-T and CAR-NK development as CD19+ target cells for cytotoxicity, killing, and potency assays
- Immunophenotyping panel design, optimisation, and instrument standardisation
- Drug screening and mechanism-of-action work for B-cell-depleting antibodies, bispecific engagers, and BTK inhibitors
- Baseline reference material for comparison against disease-state B cell products
Other Healthy Immune Cell Subsets
Compliance and Quality Assurance
- IRB-approved collections with 21 CFR Part 11-compliant e-consent
- Standardized processing and cryopreservation protocols
- HIPAA-compliant donor data protection
Ordering & Customization
Healthy CD19 B Cells are available in standard or custom aliquot sizes. For pricing, international shipping, documentation, or regulatory support, please contact learnmore@sanguinebio.com. To browse the full range of isolated B cell products and current availability, visit our human CD19+ B cells page.
Applications
- Immunology and translational research
- Biomarker discovery and validation
- Drug screening and assay development
- Vaccine and infectious disease research
Product Features
- Research Use Only (RUO), CD19+ B Cells
- Sourced from screened, consented donors
- Processed under validated SOPs
- QC tested for purity, viability, and sterility
- Processed within 24 hours of collection
- IRB-approved protocols with electronic informed consent
Donor Metadata
- Verified diagnosis and clinical history
- Demographic data: age, sex assigned at birth, race/ethnicity
- Medication and treatment background when available
Compliance and Quality Assurance
- IRB-approved collections and standardized procedures
- 21 CFR Part 11-compliant e-consent system
- HIPAA-compliant data management
Ordering & Customization
Human CD19+ B Cells are available in customizable aliquot sizes. For international orders or documentation needs, please contact learnmore@sanguinebio.com to confirm requirements and availability. We also work with multiple ordering platforms and distributors, learn more here.
Protocols & Documentation
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Download
Immune Cell Isolation
Guidelines for isolating immune cell populations with validated enrichment steps, purity checkpoints, and documentation.
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Informed Consent Form (ICF)
Available upon request — confirms donor consent for research use and downstream commercialization.
CD19+ B Cells
Where can I find your complete catalog?
You can find our full product catalog here.
How long can samples be stored?
Storage depends on sample type but often cryopreserved samples stored in liquid nitrogen can be stored for years.
What customization options are available?
Extensive customization is available. To discuss your project request a quote or email us at learnmore@sanguinebio.com.
Do you offer prospective collections?
Sanguine offers flexible prospective collection services tailored to fit your research. For more information and to request a quote, please see: our prospective biospecimen collection services page.
What is the turnaround time for custom collections?
Timelines depend on condition, sample type, and I/E criteria but we often start collection within 2 weeks of signed agreement.
Do you have samples in stock?
YES - we have in-stock inventory. You can also email us at learnmore@sanguinebio.com as we continually get new samples in our inventory.
What does unique donor mean?
Each sample from a different individual - Unique donor means each sample comes from a different person, ensuring biological diversity in your study.
Are samples collected under IRB-approved protocols?
YES - "IRB-approved collection protocols" and IRB approval documentation available upon request. Sanguine utilizes two internationally-recognized IRBs (Advarra and WCG IRB) for review and approval.
Frequently Asked Questions
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Need a custom CD19+ B Cells cohort?
Our scientific team can scope prospective collections with donor-specific I/E criteria, typically starting within 2 weeks of agreement.