CD3+ T Cells

High-Purity Human CD3+ T Cells for Immunology and Cell Therapy Research

Human CD3+ T cells are purified from the peripheral blood of IRB-consented donors under IRB-approved protocols and cryopreserved in CryoStor® CS10 media. These isolated CD3+ T cells support T cell biology, cancer immunotherapy, autoimmune disease research, TCR-based drug development, and other human T cell research applications, delivering high viability and consistent performance for in vitro and in vivo studies. Buy disease-state CD3+ T cell samples here.

What CD3+ Selection Gives You

CD3 is not a lineage marker of convenience — it is the signal-transducing core of the T cell receptor complex. Selecting on CD3 therefore captures the functional T cell compartment as a whole, which determines what these cells can and cannot be used for.

• CD3+ isolation yields the total mature T cell pool: CD4+ helper, CD8+ cytotoxic, regulatory, memory and naive subsets, and unconventional populations such as gamma-delta and NKT-like T cells, preserved in their donor-native proportions.
• Because subset ratios are retained, CD3+ preparations are the right starting point when the readout depends on T cell composition; single-subset work is better served by CD4+ or CD8+ isolations.
• The CD3 complex is the target of anti-CD3 antibody stimulation, so CD3+ cells respond to standard polyclonal activation with anti-CD3 and anti-CD28 without further manipulation.
• CD3+ T cells are the conventional starting material for CAR-T and TCR-T engineering, where transduction and expansion efficiency are assessed against a defined input population.
• Cryopreservation in CryoStor® CS10, a defined serum-free medium, supports reproducible post-thaw viability and reduces the lot-to-lot variability introduced by serum-containing freeze media.
• Isolating T cells within 24 hours of collection limits ex vivo activation and apoptosis, so the recovered cells better reflect the donor’s circulating T cell state.

Donor Stratification Available

• Healthy donors or clinically confirmed disease-state donors across autoimmune, inflammatory, infectious, and oncology cohorts
• Age band and sex assigned at birth for demographic matching
• Race and ethnicity
• HLA typing available on request for selected inventory
• Viral serostatus, including CMV, where documented
• Donor-reported medication and treatment status for disease-state cohorts
• Matched healthy controls available alongside any disease-state cohort

Product Features

• Human CD3+ T cells for Research Use Only (RUO)
• Isolated human CD3+ T cell samples from clinically screened donors
• High-purity isolated CD3+ T cells with ≥90% CD3+ viability
• Cryopreserved in CryoStor® CS10 media
• Processed within 24 hours of collection
• IRB-approved protocols with electronic informed consent
• Cell counts and purity reported per lot; custom aliquots available on request

De-identified Donor Data

• Comprehensive donor screening and verified health or diagnosis status
• Demographic data: age, sex assigned at birth, race/ethnicity
• Self-reported medication, allergy, and infectious disease history
• Additional donor metadata available on request

Applications

• T cell activation, proliferation, and cytokine release assays
• CAR-T and TCR-T therapy development, transduction, and expansion
• T cell exhaustion, memory differentiation, and phenotyping studies
• Autoimmune, inflammatory, and infectious disease modelling
• Bispecific antibody and T cell engager potency and redirected cytotoxicity assays
• Single-cell RNA sequencing and TCR repertoire analysis
• Mixed lymphocyte reactions and allogeneic response studies
• Immunosuppressant and immunomodulator screening

Other Human Immune Cell and Blood Products

• Human CD4+ T Cells
• Human CD8+ T Cells
• Human CD19+ B Cells
• Human CD56+ NK Cells
• Human PBMCs
• Human Leukopak

Compliance and Quality Assurance

• IRB-approved collections and standardized procedures
• 21 CFR Part 11–compliant e-consent system
• HIPAA-compliant data management

Buy Human CD3+ T Cells for Research

Purchase human T cell samples for research, including isolated CD3+ T cells from healthy or disease-state donors, with flexible aliquots and documentation support.

Ordering & Customization

Purchase human CD3+ T cells in standard or custom aliquots. For pricing, availability, international orders, or regulatory compliance documentation, please contact learnmore@sanguinebio.com. If you need to buy CD3+ T cells for research with specific donor criteria, Sanguine can support custom sourcing and documentation support. Cells can also be isolated to order from our human leukopak inventory.