Human CD4+ T Cells for Research

Human CD4+ T Cells for Research

Source human CD4+ T cells and CD4 T cell samples for immunology, cell therapy, and T cell biology research. Healthy and disease-state donor options are available.

Human CD4+ T cells are purified from peripheral blood of IRB-consented donors under IRB-approved protocols. As the helper arm of the adaptive immune system, CD4+ T cells recognise antigen presented on MHC class II and coordinate the response of B cells, cytotoxic T cells, and macrophages through cytokine signalling. That central regulatory role makes them the workhorse population for autoimmune research, vaccine and adjuvant studies, immune-modulator screening, and cell therapy development. Available cryopreserved, these CD4+ T cells deliver high viability and consistent performance for in vitro and in vivo studies.

Buy CD4+ T cells from healthy and disease-state donors.

CD4+ T Cell Subsets and Phenotyping

Because helper T cells are functionally heterogeneous, most studies need a defined subset rather than bulk CD4+ cells. Subset enrichment and phenotyping data are available on request:

• Naive (CD45RA+ CCR7+) and memory (CD45RO+) compartments
• Central memory and effector memory populations
• Regulatory T cells (Treg, CD4+ CD25+ CD127low FoxP3+)
• Th1, Th2, and Th17 polarisation-competent starting material
• HLA-typed donors for antigen-specific and MHC class II–restricted work

Isolation and Purity

CD4+ T cells are isolated from fresh peripheral blood or apheresis material by immunomagnetic selection, then cryopreserved to preserve function. Negative selection is used where an untouched, label-free population is required for downstream functional assays.

• High-purity isolated CD4+ T cells with ≥90% CD4+ viability
• Flow cytometry purity and viability data supplied per lot
• Cryopreserved in CryoStor® CS10 media
• Processed within 24 hours of collection

Product Features

• Human CD4+ T cells for Research Use Only (RUO)
• CD4 positive T cells
• Isolated human CD4+ T cell samples from healthy, clinically screened donors
• Disease-state donor cohorts available to specification
• Custom aliquot sizes and repeat draws from the same donor where available
• IRB-approved protocols with electronic informed consent

Donor Metadata

• Comprehensive donor screening and verified health status
• Demographic data: age, sex assigned at birth, race/ethnicity
• Self-reported medication, allergy, and infectious disease history
• HLA type and CMV serostatus available on request

Applications

• T helper cell activation and cytokine secretion assays
• Th1, Th2, Th17, and Treg differentiation studies
• Treg suppression assays and immune tolerance research
• CAR-T and TCR engineering, including immune interaction modeling
• Autoimmune and inflammatory disease research
• HIV and infectious disease studies
• Single-cell sequencing and transcriptomic profiling

Disease-State CD4+ T Cells

CD4+ T cells are available from donors with physician-confirmed diagnoses, which is essential when the helper compartment itself is the disease mechanism. Current options include systemic lupus erythematosus CD4+ T cells and scleroderma CD4+ T cells, alongside healthy donor CD4+ T cells as matched controls. Other indications can be sourced as a custom collection.

Related Cell Types

If you need a different fraction of the same donor material, see our human CD8+ T cells, human CD3+ T cells (pan-T), or the unseparated human PBMCs they are derived from. For large-scale isolation, a human leukopak yields substantially more cells per donor.

Compliance and Quality Assurance

• IRB-approved collections and standardized procedures
• 21 CFR Part 11–compliant e-consent system
• HIPAA-compliant data management

Buy Human CD4+ T Cells for Research

Purchase human CD4+ T cell samples for research, including isolated CD4+ T cells from healthy or disease-state donors, with flexible aliquots and documentation support. For a sourcing and quality overview, read our guide to buying frozen CD4+ T cells.

Frequently Asked Questions

What purity can I expect? Isolated CD4+ T cells are supplied at ≥90% purity and viability, with per-lot flow cytometry data.

Can I get Tregs specifically? Yes — CD4+ CD25+ CD127low regulatory T cells can be enriched on request, and FoxP3 confirmation is available.

Are matched CD8+ or PBMC fractions available from the same donor? Yes. Matched fractions from a single collection can be provided where the draw volume allows, which is the cleanest design for comparative work.

Do you supply fresh rather than cryopreserved CD4+ T cells? Fresh isolation is possible for customers within logistics range; contact us to confirm timing against your assay schedule.

Ordering & Customization

Purchase human CD4+ T cells in standard or custom aliquots. For international orders or regulatory compliance documentation, please contact learnmore@sanguinebio.com. If you need to buy CD4+ T cells for research with specific donor criteria, Sanguine can support custom sourcing and documentation support.