Nonalcoholic Fatty Liver Disease PBMC

About Nonalcoholic Fatty Liver Disease PBMC

Nonalcoholic Fatty Liver Disease PBMC for Metabolic Inflammation Research

Nonalcoholic Fatty Liver Disease PBMC products are sourced from IRB-consented donors clinically diagnosed with nonalcoholic fatty liver disease (NAFLD, also described as MASLD) and processed within 24 hours of collection under standardized SOPs. These cryopreserved peripheral blood mononuclear cells are stored in CryoStor® CS10 and shipped on dry ice. Research Use Only (RUO).

Metabolic Inflammation and Why PBMC

• NAFLD is a metabolic, not autoimmune, liver disease. It begins with hepatic steatosis, the accumulation of triglyceride in hepatocytes, in the absence of significant alcohol intake.
• The dominant drivers are insulin resistance, obesity, and excess free fatty acid flux to the liver, together with increased de novo lipogenesis. NAFLD is closely linked to type 2 diabetes, dyslipidemia, and the broader cardiometabolic phenotype.
• Lipotoxicity from specific lipid species, rather than neutral triglyceride itself, causes hepatocyte stress and injury, and this is what distinguishes progressive steatohepatitis from bland steatosis.
• Progression to nonalcoholic steatohepatitis involves hepatocyte ballooning, lobular inflammation, and fibrosis, with recruitment of circulating monocytes that differentiate into monocyte-derived hepatic macrophages alongside resident Kupffer cells.
• Because these liver macrophages originate in the peripheral blood monocyte pool, PBMCs provide a directly relevant, accessible source of the innate cells implicated in disease progression, as well as lymphocyte populations altered in metabolic disease.
• PBMCs support live-cell immunophenotyping, monocyte and macrophage differentiation models, lipid loading experiments, immunometabolic assays, and single-cell transcriptomics that acellular matrices cannot.

Donor Stratification Available

• Disease stage: simple steatosis versus nonalcoholic steatohepatitis where documented
• Fibrosis stage or non-invasive fibrosis score where available
• Biopsy-confirmed versus imaging or clinically diagnosed donors
• Type 2 diabetes and insulin resistance status
• BMI category and dyslipidemia status
• Treatment status, including GLP-1 receptor agonist, insulin sensitizer, or lifestyle management only
• Matched healthy controls available

Product Features

• Research Use Only (RUO), cryopreserved PBMCs
• Clinically confirmed NAFLD donors
• Processed within 24 hours of collection
• Stored using CryoStor® CS10 freezing media
• Custom aliquots available; shipped on dry ice
• IRB-approved protocols and electronic informed consent
• Screen LeukoLot™ available prior to bulk orders

De-identified Donor Data

• Verified NAFLD diagnosis
• Donor demographics: age, sex assigned at birth, race/ethnicity
• Self-reported allergies and infectious disease history
• BMI, metabolic comorbidities, and liver enzyme values where available
• Additional NAFLD-specific data available upon request

Applications

• Monocyte subset immunophenotyping and monocyte-derived macrophage differentiation models
• Immunometabolism assays, including mitochondrial and glycolytic function
• Lipid loading and lipotoxicity models in primary human cells
• Ex vivo stimulation and inflammatory cytokine release profiling
• Bulk and single-cell transcriptomic analysis of metabolic inflammation
• Target validation and mechanism-of-action work for NASH and metabolic therapeutics
• Peripheral immune correlate discovery for fibrosis progression
• Drug screening in disease-state primary cells

Other Nonalcoholic Fatty Liver Disease Specimen Types

• Nonalcoholic Fatty Liver Disease Plasma
• Nonalcoholic Fatty Liver Disease Serum
• Nonalcoholic Fatty Liver Disease Whole Blood
• Nonalcoholic Fatty Liver Disease Leukopak
• Nonalcoholic Fatty Liver Disease Bulk Plasma

Compliance and Quality Assurance

• IRB-approved collections and protocols
• 21 CFR Part 11-compliant e-consent system
• HIPAA-compliant donor data protection

Ordering & Customization

Nonalcoholic Fatty Liver Disease PBMC samples are shipped on dry ice and available in custom aliquots. For pricing, availability, international orders, or regulatory requirements, please contact learnmore@sanguinebio.com to confirm documentation and compliance needs. To browse all donor cohorts and current stock, visit our human PBMCs page.