Pemphigoid PBMC

About Pemphigoid PBMC

Pemphigoid PBMC for Autoimmune Blistering Disease Research

Pemphigoid PBMC samples are collected from IRB-consented donors clinically diagnosed with pemphigoid and processed within 24 hours of collection under controlled conditions. Pemphigoid is a genuinely autoantibody-mediated disease of the dermal-epidermal junction, and cryopreserved peripheral blood mononuclear cells (PBMCs) provide the autoreactive B- and T-cell material needed to interrogate how those autoantibodies arise and are sustained.

Pemphigoid Immunology and Why PBMCs

• Pemphigoid is driven by IgG autoantibodies against hemidesmosomal proteins – principally BP180 (collagen XVII, the transmembrane component) and BP230 (a plakin-family intracellular protein) – which anchor basal keratinocytes to the underlying basement membrane.
• Autoantibody binding at the dermal-epidermal junction activates complement and recruits neutrophils, eosinophils, and mast cells, whose proteases cleave the anchoring complex and produce the subepidermal blister that defines the disease histologically.
• Anti-BP180 titres, particularly against the immunodominant NC16A domain, correlate reasonably well with disease activity and are the standard serological readout; anti-BP230 is a useful complementary marker.
• IgE autoantibodies against BP180 and prominent tissue eosinophilia implicate a type 2 immune component alongside the IgG response, which has motivated interest in IL-4/IL-13- and eosinophil-directed therapies.
• Autoreactive B cells require T-follicular helper support to undergo class switching and affinity maturation, so PBMCs are the specimen of choice for antigen-specific B-cell identification, BCR repertoire work, and T-cell help assays.
• PBMCs support flow cytometric immunophenotyping and functional assays that cannot be performed on plasma or serum, making them complementary to the acellular pemphigoid products.

Donor Stratification Available

• Clinical variant: bullous pemphigoid versus mucous membrane pemphigoid, where documented
• Serology status: anti-BP180 and anti-BP230 results where testing is documented
• Disease activity at collection: active blistering versus clinical remission
• Treatment exposure: treatment-naive, topical or systemic corticosteroid, steroid-sparing immunosuppressant, rituximab, or biologic treated
• Presence of associated eosinophilia or elevated total IgE where documented
• Age band, sex, and relevant comorbidities including neurological disease and diabetes
• Matched healthy controls available

Product Features

• Research Use Only (RUO), cryopreserved pemphigoid PBMC samples
• Clinically confirmed pemphigoid donors
• Available in cell counts ranging from 5 million to 2 billion
• Processed within 24 hours of collection
• Preserved using CryoStor® CS10 freezing media
• IRB-approved protocols and electronic informed consent
• Screening (LeukoLot™) available prior to bulk orders

De-identified Donor Data

• Verified pemphigoid diagnosis
• Donor demographics: age, sex assigned at birth, race/ethnicity
• Self-reported allergies and infectious disease history
• Serology results, disease activity, and treatment history where documented
• Additional pemphigoid-specific clinical data available on request

Applications

• Immunophenotyping of B-cell, plasmablast, and T-follicular helper compartments by flow or spectral cytometry
• Antigen-specific B-cell identification and sorting using BP180 and BP230 tetramers or probes
• B-cell receptor repertoire sequencing and monoclonal antibody cloning from autoreactive clones
• T-cell help and autoantigen-specific T-cell response assays
• Single-cell RNA sequencing and CITE-seq of the circulating immune compartment
• Ex vivo drug screening and mechanism-of-action work for B-cell-depleting and type 2-directed therapeutics
• Transcriptomic and proteomic profiling of sorted immune subsets
• Biomarker discovery and validation for disease activity and relapse prediction

Other Pemphigoid Specimen Types

• Pemphigoid Plasma
• Pemphigoid Serum
• Pemphigoid Whole Blood
• Pemphigoid Leukopak
• Bullous Pemphigoid Skin Punch Biopsy

Compliance and Quality Assurance

• IRB-approved collections and protocols
• 21 CFR Part 11-compliant e-consent
• HIPAA-compliant donor data protection

Ordering & Customization

Pemphigoid PBMC samples ship on dry ice in standard or custom aliquots. For pricing, international orders, or regulatory documentation, contact learnmore@sanguinebio.com. Browse all human PBMCs or request a custom disease-state collection.