Skin Cancer PBMC

About Skin Cancer PBMC

Skin Cancer PBMC for Immuno-Oncology Research

Skin Cancer PBMC samples are cryopreserved peripheral blood mononuclear cells from IRB-consented donors with a clinically confirmed skin cancer diagnosis. Cells are processed within 24 hours of collection and preserved in CryoStor® CS10 to support tumour immunology research across melanoma, basal cell carcinoma, and cutaneous squamous cell carcinoma.

Skin Cancer Tumour Immunology and Why PBMC

Skin cancer is a neoplastic disease. Its subtypes arise from different cells of origin and carry different mutational and immunological profiles, and the systemic immune compartment is central to how they are now treated:

• Ultraviolet radiation is the dominant carcinogen, leaving a characteristic UV mutational signature and giving melanoma and cutaneous squamous cell carcinoma among the highest tumour mutational burdens of any cancer
• Subtype-defining drivers differ: BRAF and NRAS mutations in melanoma, hedgehog pathway activation through PTCH1 in basal cell carcinoma, and TP53 with high UV-driven mutation load in squamous cell carcinoma
• High neoantigen load makes these tumours immunogenic, which is why immune checkpoint blockade against PD-1, PD-L1, and CTLA-4 has been transformative in advanced melanoma and cutaneous squamous cell carcinoma
• Systemic T-cell state matters. Circulating T-cell subsets, exhaustion markers such as PD-1, TIM-3, and LAG-3, and myeloid populations are studied as correlates of checkpoint inhibitor response and immune-related adverse events
• Immunosuppression is a risk factor — solid organ transplant recipients have markedly increased squamous cell carcinoma incidence, underlining the role of immune surveillance in cutaneous carcinogenesis
• Why PBMC: immunophenotyping, T-cell receptor repertoire sequencing, antigen-specific T-cell expansion, ex vivo checkpoint blockade, and single-cell profiling are all performed on cryopreserved patient PBMCs

Donor Stratification Available

• Histological subtype: melanoma, basal cell carcinoma, or cutaneous squamous cell carcinoma
• Stage and extent of disease, including localised versus metastatic
• Treatment status: treatment-naive, post-surgical, or on checkpoint inhibitor or targeted therapy
• Treatment response category and progression status where documented
• Immunosuppressed donors, including transplant recipients, where available
• Age, sex assigned at birth, race/ethnicity, and Fitzpatrick skin type
• Matched healthy controls available

Product Features

• Research Use Only (RUO), cryopreserved skin cancer PBMC samples
• Clinically confirmed skin cancer donors, with histological subtype annotated
• Available in cell counts ranging from 5 million to 2 billion
• Processed within 24 hours of collection
• Preserved using CryoStor® CS10 freezing media
• IRB-approved protocols and electronic informed consent
• Screening (LeukoLot™) available prior to bulk orders

De-identified Donor Data

• Verified skin cancer diagnosis
• Donor demographics: age, sex assigned at birth, race/ethnicity
• Self-reported allergies and infectious disease history
• Additional skin cancer subtype, staging, and treatment data available on request

Applications

• Immunophenotyping of T-cell, NK, and myeloid populations in skin cancer
• T-cell exhaustion and checkpoint receptor expression profiling
• Ex vivo checkpoint blockade and immune agonist pharmacology
• T-cell receptor repertoire sequencing and clonality analysis
• Antigen-specific T-cell expansion and functional cytotoxicity assays
• Cell therapy and engineered T-cell process development using patient-derived cells
• Single-cell RNA sequencing and multi-omic immune profiling
• Biomarker discovery for immunotherapy response and immune-related toxicity

Other Skin Cancer Specimen Types

• Skin Cancer Leukopak — apheresis-derived, for high cell-number requirements
• Skin Cancer Serum — for circulating protein and cytokine biomarkers
• Skin Cancer Whole Blood — fresh, for same-day assays and genotyping
• Melanoma PBMC — for a melanoma-specific cohort
• Solid Tumor Cancer PBMC — for broader oncology cohorts

Compliance and Quality Assurance

• IRB-approved collections and protocols
• 21 CFR Part 11-compliant e-consent
• HIPAA-compliant donor data protection

Ordering & Customization

Skin Cancer PBMC samples ship on dry ice in standard or custom aliquots. For pricing, international orders, or regulatory documentation, contact learnmore@sanguinebio.com. Browse all human PBMCs or request a custom disease-state collection.