Acne PBMC
Acne PBMC from clinically confirmed acne donors, cryopreserved in CryoStor® CS10 within 24 hours of collection. Suitable for innate immune characterisation, Cutibacterium acnes and TLR ligand stimulation assays, IL-1 beta and inflammasome studies, and Th17/IL-17 response work in inflammatory dermatology.
Acne PBMC for Inflammatory Dermatology Research
Human Acne PBMC are isolated from the peripheral blood of IRB-consented donors with a clinically confirmed diagnosis of acne vulgaris, cryopreserved in CryoStor® CS10 and processed within 24 hours of collection. These peripheral blood mononuclear cells provide a viable immune cell population for interrogating the innate and Th17 responses that drive inflammatory acne lesions.
Inflammatory Mechanisms in Acne Vulgaris
Acne vulgaris is a chronic inflammatory disorder of the pilosebaceous unit. It is not an autoimmune disease and has no associated autoantibody; the pathology follows from sebum production, follicular keratinisation and the host innate immune response to commensal bacteria within the follicle.
- Four processes interact in acne: increased androgen-driven sebum production, follicular hyperkeratinisation and comedo formation, proliferation of Cutibacterium acnes within the follicle, and the inflammatory response this provokes.
- C. acnes is recognised by innate immune receptors including TLR2, and the resulting signalling drives IL-1 beta, IL-8 and TNF production — an innate response directly testable in mononuclear cells.
- Inflammasome activation and IL-1 beta release are established features of the acne inflammatory cascade, providing a mechanistically defined ex vivo stimulation endpoint.
- C. acnes also induces Th17 differentiation and IL-17 production, which is why an isolated mononuclear population is a legitimate model system rather than a proxy for skin.
- PBMC allow donor-derived immune cells to be challenged with C. acnes or defined ligands and the cytokine response quantified against matched controls.
- Because acne is a localised follicular disease, PBMC characterise the systemic immune response and its reactivity rather than lesional pathology itself; skin biopsy material is the appropriate complement for lesional work.
Donor Stratification Available
- Severity: mild, moderate, or severe/nodulocystic acne where documented
- Lesion type predominance: comedonal, inflammatory papulopustular, or nodulocystic
- Anatomical distribution: facial, truncal, or both
- Treatment status: topical retinoid, topical or systemic antibiotic, isotretinoin, hormonal therapy, or treatment-naive
- Age group: adolescent vs adult-onset acne
- Scarring or post-inflammatory pigmentation where documented
- Matched healthy controls available
Product Features
- Research Use Only (RUO), cryopreserved PBMCs
- Clinically confirmed acne donors
- Available in cell counts ranging from 5 million to 2 billion
- Processed within 24 hours of collection
- Stored using CryoStor® CS10 freezing media
- Viability and recovery reported per lot
- IRB-approved protocols and electronic informed consent
- Screen LeukoLot™ available prior to bulk orders
De-identified Donor Data
- Verified acne diagnosis and severity grading where documented
- Donor demographics: age, sex assigned at birth, race/ethnicity
- Current and prior acne treatment history
- Self-reported allergies and infectious disease history
- Additional acne-specific data available upon request
Applications
- Innate immune cell characterisation and immunophenotyping in acne donors
- Cutibacterium acnes and TLR ligand ex vivo stimulation assays
- IL-1 beta and inflammasome pathway studies
- Th17 differentiation and IL-17 production analysis
- Monocyte activation and cytokine secretion profiling
- Transcriptomic and single-cell profiling of circulating immune cells
- Screening and target validation for anti-inflammatory and anti-IL-17 dermatology candidates
- Biomarker discovery for acne severity and treatment response
Other Acne Specimen Types
Compliance and Quality Assurance
- IRB-approved collections and protocols
- 21 CFR Part 11-compliant e-consent system
- HIPAA-compliant donor data protection
- Documentation support available for regulatory review
Ordering & Customization
Acne PBMC samples are shipped on dry ice and available in custom aliquots. For pricing, current availability, international orders or regulatory documentation, contact learnmore@sanguinebio.com. To browse disease-state options and live stock status across our full PBMC inventory, visit our human PBMC page.
Protocols & Documentation
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PBMC Isolation from Whole Blood
Standard operating procedure for isolating peripheral blood mononuclear cells from whole blood using density-gradient separation.
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Immune Cell Isolation
Guidelines for isolating immune cell populations with validated enrichment steps, purity checkpoints, and documentation.
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Choosing the Right PBMC Configuration
Reference guide comparing PBMC formats, cryopreservation states, and study-fit recommendations for your application.
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Thawing Cryopreserved PBMC
Validated thawing protocol to recover viable cryopreserved PBMCs while minimizing activation and loss of function.
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Informed Consent Form (ICF)
Available upon request — confirms donor consent for research use and downstream commercialization.
PBMC
Frequently Asked Questions
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YES – All collections are conducted under IRB-approved protocols and electronic informed consent. Sanguine utilizes Advarra and WCG IRB for oversight.
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YES – all our products are research use only (RUO).
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You can find our full product catalog here.
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Storage depends on sample type but often cryopreserved samples stored in liquid nitrogen can be stored for years.
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Extensive customization is available. To discuss your project request a quote or email us at learnmore@sanguinebio.com.
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Sanguine offers flexible prospective collection services tailored to fit your research. For more information and to request a quote, please see: our prospective biospecimen collection services page.
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Timelines depend on condition, sample type, and I/E criteria but we often start collection within 2 weeks of signed agreement.
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YES – we have in-stock inventory. You can also email us at learnmore@sanguinebio.com as we continually get new samples in our inventory.
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Each sample from a different individual – Unique donor means each sample comes from a different person, ensuring biological diversity in your study.
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YES – Unique donors can be specified and guaranteed based on your requirements.
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For information about sample quality, please see: Quality and Compliance
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YES – “IRB-approved collection protocols” and IRB approval documentation available upon request. Sanguine utilizes two internationally-recognized IRBs (Advarra and WCG IRB) for review and approval.
Ask a Question
Need a custom PBMC cohort?
Our scientific team can scope prospective collections with donor-specific I/E criteria, typically starting within 2 weeks of agreement.