Acne PBMC
Powered by BiozAbout:Acne PBMC from clinically confirmed acne donors, cryopreserved in CryoStor® CS10 within 24 hours of collection. Suitable for innate immune characterisation, Cutibacterium acnes and TLR ligand stimulation assays, IL-1 beta and inflammasome studies, and Th17/IL-17 response work in inflammatory dermatology.
About Acne PBMC
Acne PBMC for Inflammatory Dermatology Research
Human Acne PBMC are isolated from the peripheral blood of IRB-consented donors with a clinically confirmed diagnosis of acne vulgaris, cryopreserved in CryoStor® CS10 and processed within 24 hours of collection. These peripheral blood mononuclear cells provide a viable immune cell population for interrogating the innate and Th17 responses that drive inflammatory acne lesions.
Inflammatory Mechanisms in Acne Vulgaris
Acne vulgaris is a chronic inflammatory disorder of the pilosebaceous unit. It is not an autoimmune disease and has no associated autoantibody; the pathology follows from sebum production, follicular keratinisation and the host innate immune response to commensal bacteria within the follicle.
- Four processes interact in acne: increased androgen-driven sebum production, follicular hyperkeratinisation and comedo formation, proliferation of Cutibacterium acnes within the follicle, and the inflammatory response this provokes.
- C. acnes is recognised by innate immune receptors including TLR2, and the resulting signalling drives IL-1 beta, IL-8 and TNF production — an innate response directly testable in mononuclear cells.
- Inflammasome activation and IL-1 beta release are established features of the acne inflammatory cascade, providing a mechanistically defined ex vivo stimulation endpoint.
- C. acnes also induces Th17 differentiation and IL-17 production, which is why an isolated mononuclear population is a legitimate model system rather than a proxy for skin.
- PBMC allow donor-derived immune cells to be challenged with C. acnes or defined ligands and the cytokine response quantified against matched controls.
- Because acne is a localised follicular disease, PBMC characterise the systemic immune response and its reactivity rather than lesional pathology itself; skin biopsy material is the appropriate complement for lesional work.
Donor Stratification Available
- Severity: mild, moderate, or severe/nodulocystic acne where documented
- Lesion type predominance: comedonal, inflammatory papulopustular, or nodulocystic
- Anatomical distribution: facial, truncal, or both
- Treatment status: topical retinoid, topical or systemic antibiotic, isotretinoin, hormonal therapy, or treatment-naive
- Age group: adolescent vs adult-onset acne
- Scarring or post-inflammatory pigmentation where documented
- Matched healthy controls available
Product Features
- Research Use Only (RUO), cryopreserved PBMCs
- Clinically confirmed acne donors
- Available in cell counts ranging from 5 million to 2 billion
- Processed within 24 hours of collection
- Stored using CryoStor® CS10 freezing media
- Viability and recovery reported per lot
- IRB-approved protocols and electronic informed consent
- Screen LeukoLot™ available prior to bulk orders
De-identified Donor Data
- Verified acne diagnosis and severity grading where documented
- Donor demographics: age, sex assigned at birth, race/ethnicity
- Current and prior acne treatment history
- Self-reported allergies and infectious disease history
- Additional acne-specific data available upon request
Applications
- Innate immune cell characterisation and immunophenotyping in acne donors
- Cutibacterium acnes and TLR ligand ex vivo stimulation assays
- IL-1 beta and inflammasome pathway studies
- Th17 differentiation and IL-17 production analysis
- Monocyte activation and cytokine secretion profiling
- Transcriptomic and single-cell profiling of circulating immune cells
- Screening and target validation for anti-inflammatory and anti-IL-17 dermatology candidates
- Biomarker discovery for acne severity and treatment response
Other Acne Specimen Types
Compliance and Quality Assurance
- IRB-approved collections and protocols
- 21 CFR Part 11-compliant e-consent system
- HIPAA-compliant donor data protection
- Documentation support available for regulatory review
Ordering & Customization
Acne PBMC samples are shipped on dry ice and available in custom aliquots. For pricing, current availability, international orders or regulatory documentation, contact learnmore@sanguinebio.com. To browse disease-state options and live stock status across our full PBMC inventory, visit our human PBMC page.
Applications
- Immunology and translational research
- Biomarker discovery and validation
- Drug screening and assay development
- Vaccine and infectious disease research
Product Features
- Research Use Only (RUO), PBMC
- Sourced from screened, consented donors
- Processed under validated SOPs
- QC tested for purity, viability, and sterility
- Processed within 24 hours of collection
- IRB-approved protocols with electronic informed consent
Donor Metadata
- Verified diagnosis and clinical history
- Demographic data: age, sex assigned at birth, race/ethnicity
- Medication and treatment background when available
Compliance and Quality Assurance
- IRB-approved collections and standardized procedures
- 21 CFR Part 11-compliant e-consent system
- HIPAA-compliant data management
Ordering & Customization
Human PBMC are available in customizable aliquot sizes. For international orders or documentation needs, please contact learnmore@sanguinebio.com to confirm requirements and availability. We also work with multiple ordering platforms and distributors, learn more here.
Protocols & Documentation
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PBMC Isolation from Whole Blood
Standard operating procedure for isolating peripheral blood mononuclear cells from whole blood using density-gradient separation.
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Immune Cell Isolation
Guidelines for isolating immune cell populations with validated enrichment steps, purity checkpoints, and documentation.
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Choosing the Right PBMC Configuration
Reference guide comparing PBMC formats, cryopreservation states, and study-fit recommendations for your application.
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Thawing Cryopreserved PBMC
Validated thawing protocol to recover viable cryopreserved PBMCs while minimizing activation and loss of function.
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Informed Consent Form (ICF)
Available upon request — confirms donor consent for research use and downstream commercialization.
PBMC
Where can I find your complete catalog?
You can find our full product catalog here.
How long can samples be stored?
Storage depends on sample type but often cryopreserved samples stored in liquid nitrogen can be stored for years.
What customization options are available?
Extensive customization is available. To discuss your project request a quote or email us at learnmore@sanguinebio.com.
Do you offer prospective collections?
Sanguine offers flexible prospective collection services tailored to fit your research. For more information and to request a quote, please see: our prospective biospecimen collection services page.
What is the turnaround time for custom collections?
Timelines depend on condition, sample type, and I/E criteria but we often start collection within 2 weeks of signed agreement.
Do you have samples in stock?
YES - we have in-stock inventory. You can also email us at learnmore@sanguinebio.com as we continually get new samples in our inventory.
What does unique donor mean?
Each sample from a different individual - Unique donor means each sample comes from a different person, ensuring biological diversity in your study.
Are samples collected under IRB-approved protocols?
YES - "IRB-approved collection protocols" and IRB approval documentation available upon request. Sanguine utilizes two internationally-recognized IRBs (Advarra and WCG IRB) for review and approval.
Frequently Asked Questions
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Pair with a Healthy Control
Match your Acne PBMC with normal donor samples for comparison
Healthy Donor PBMCs
705 In Stock View InventoryNeed a custom PBMC cohort?
Our scientific team can scope prospective collections with donor-specific I/E criteria, typically starting within 2 weeks of agreement.