Alpha-Gal Syndrome Leukopak
LeukoCore Alpha-Gal Syndrome Leukopak products contain concentrated immune cells from IRB-consented donors with clinically confirmed alpha-gal syndrome (AGS), an IgE-mediated allergy to galactose-alpha-1,3-galactose acquired after tick-bite sensitisation. Ideal for high-yield basophil activation testing, antigen-specific T cell work, immunophenotyping and single-cell studies. Matched healthy controls available.
Alpha-Gal Syndrome Leukopak for Allergy and Type 2 Immunity Research
LeukoCore Alpha-Gal Syndrome Leukopak biospecimens are collected from IRB-consented donors clinically diagnosed with alpha-gal syndrome (AGS) through IRB-approved apheresis procedures. Each leukopak contains large quantities of peripheral blood mononuclear cells, processed and shipped within 24 hours of collection, making it suitable for high-cell-demand allergy immunology and drug development work.
Alpha-Gal Sensitisation and Why a Leukopak
Alpha-gal syndrome is an IgE-mediated, type 2 allergic condition. It is not autoimmune. Sensitisation is to a carbohydrate rather than a protein, which makes it mechanistically distinct from most food allergies.
- The target allergen is galactose-alpha-1,3-galactose (alpha-gal), an oligosaccharide present in non-primate mammalian tissue but absent in humans
- Sensitisation follows tick bites, with specific IgE to alpha-gal developing after exposure; in the United States the lone star tick is the principal implicated species
- Subsequent ingestion of mammalian meat produces a characteristically delayed allergic reaction, typically hours after eating, reflecting the time needed for lipid-associated alpha-gal to be processed and presented
- Because the epitope is a glycan, cross-reactivity extends to alpha-gal-bearing biologics and gelatin-containing products, which is directly relevant to biologic drug safety research
- Alpha-gal-specific IgE, effector cell activation via FcepsilonRI on basophils and mast cells, and type 2 T cell help are the mechanistic axes under study
- A leukopak provides the cell numbers needed for basophil activation testing, antigen-specific T cell work, cell sorting into rare subsets, and multi-omic profiling from a single donor
Donor Stratification Available
- Alpha-gal-specific IgE level band where available
- Reaction severity, including history of anaphylaxis versus cutaneous-only reactions
- Documented tick-bite exposure history
- Time since diagnosis and current dietary avoidance status
- Comorbid atopic conditions such as asthma, allergic rhinitis, or other food allergy
- Matched healthy controls available
Product Features
- Research Use Only (RUO) human leukopaks
- Clinically confirmed AGS donors
- High cell yield for large-scale studies
- Processed and shipped within 24 hours of collection
- Fresh or cryopreserved options available
- IRB-approved and ethically sourced
- Infectious disease testing
- HLA-A typing
- Screen donors with LeukoLot™
De-identified Donor Data
- Verified Alpha-Gal Syndrome (AGS) diagnosis from healthcare providers
- Donor demographics: age, sex assigned at birth, race/ethnicity
- Self-reported medication history and allergies
- Relevant clinical data including AGS severity (if available)
Applications
- Basophil activation testing with alpha-gal-bearing antigens
- Antigen-specific T cell identification and expansion
- Immune cell characterisation and deep immunophenotyping in AGS
- Cytokine profiling of type 2 responses following ex vivo stimulation
- Mast cell and effector cell precursor isolation for functional assays
- Biologic drug hypersensitivity and glycan cross-reactivity research
- Multi-omics studies including single-cell sequencing
- Therapeutic development, tolerance induction and screening
Other Alpha-Gal Syndrome Specimen Types
- Alpha-Gal Syndrome PBMC
- Alpha-Gal Syndrome Plasma
- Alpha-Gal Syndrome Serum
- Alpha-Gal Syndrome Whole Blood
- Alpha-Gal Syndrome Bulk Plasma
Compliance and Quality Assurance
- Collected under IRB oversight with donor e-consent (21 CFR Part 11 compliant)
- HIPAA-compliant handling of donor information
- Rigorous donor screening and documentation
Ordering & Customization
Alpha-Gal Syndrome Leukopak samples are available fresh or cryopreserved. For pricing, availability, or a different condition, visit our human leukopak page to browse other disease-state options and live stock status. For international shipping or regulatory documentation, please contact learnmore@sanguinebio.com to confirm regional compliance and shipping availability.
Protocols & Documentation
-
Download
Leukopak Cryopreservation
Standard operating procedure for cryopreserving leukopak units with controlled freezing and post-thaw viability targets.
-
Download
Leukopak Thawing
Validated thawing workflow for cryopreserved leukopaks to maximize recovery while maintaining sterility and chain of custody.
-
Download
Choosing the Right PBMC Configuration
Reference guide comparing PBMC formats, cryopreservation states, and study-fit recommendations for your application.
-
Informed Consent Form (ICF)
Available upon request — confirms donor consent for research use and downstream commercialization.
Leukopak
Frequently Asked Questions
1 Answer
YES – All collections are conducted under IRB-approved protocols and electronic informed consent. Sanguine utilizes Advarra and WCG IRB for oversight.
1 Answer
YES – all our products are research use only (RUO).
1 Answer
You can find our full product catalog here.
1 Answer
Storage depends on sample type but often cryopreserved samples stored in liquid nitrogen can be stored for years.
1 Answer
Extensive customization is available. To discuss your project request a quote or email us at learnmore@sanguinebio.com.
1 Answer
Sanguine offers flexible prospective collection services tailored to fit your research. For more information and to request a quote, please see: our prospective biospecimen collection services page.
1 Answer
Timelines depend on condition, sample type, and I/E criteria but we often start collection within 2 weeks of signed agreement.
1 Answer
YES – we have in-stock inventory. You can also email us at learnmore@sanguinebio.com as we continually get new samples in our inventory.
1 Answer
Each sample from a different individual – Unique donor means each sample comes from a different person, ensuring biological diversity in your study.
1 Answer
YES – Unique donors can be specified and guaranteed based on your requirements.
1 Answer
For information about sample quality, please see: Quality and Compliance
1 Answer
YES – “IRB-approved collection protocols” and IRB approval documentation available upon request. Sanguine utilizes two internationally-recognized IRBs (Advarra and WCG IRB) for review and approval.
Ask a Question
Need a custom Leukopak cohort?
Our scientific team can scope prospective collections with donor-specific I/E criteria, typically starting within 2 weeks of agreement.