Alpha-Gal Syndrome PBMC
Alpha-Gal Syndrome PBMC samples are collected from clinically confirmed donors and cryopreserved for IgE-mediated allergy research. Alpha-gal syndrome follows tick-bite sensitization to galactose-alpha-1,3-galactose and produces characteristically delayed reactions to mammalian meat. Ideal for basophil activation testing, allergen-specific T cell work, and effector-cell studies.
| Lot # | Condition | Cell Count (M) | Race/Ethnicity | Age | Gender | Country of Collection | Medications | Vials | Price | Action |
|---|---|---|---|---|---|---|---|---|---|---|
| 68262 | Alpha-Gal Syndrome (AGS) | 10M | Caucasian | 23 | Female | United States |
zoely birth control albuterol, PRN vitamin D supplement iron supplement
|
10 | $530.00 | |
| 66639 | Alpha-Gal Syndrome (AGS) | 10M | Caucasian | 55 | Male | United States |
B12/QOD Magnesium/QOD Zinc/QOD Potassium Citrate Vitamin C/QOD Lions mane/QOD
|
2 | $530.00 | |
| 66675 | Alpha-Gal Syndrome (AGS) | 10M | Caucasian | 67 | Male | United States |
Klonidine 0.1 mg QD Valsartan/HCTZ 80/25 mg QD Atorvastatin 40 mg QD Metoprolol 100 mg QD
|
8 | $530.00 | |
| 66839 | Alpha-Gal Syndrome (AGS) | 10M | Caucasian | 34 | Male | United States |
Sertraline 50 mg QD Zyrtec 20 mg BID Epi Pen PRN Pepcid 20 mg BID Singulair 10 mg QD Ativan 0.5 mg PRN
|
4 | $530.00 | |
| 66721 | Alpha-Gal Syndrome (AGS) | 10M | Caucasian | 44 | Female | United States | 3 | $530.00 |
Alpha-Gal Syndrome PBMC for IgE-Mediated Allergy Research
Human Alpha-Gal Syndrome PBMC products are sourced from donors clinically diagnosed with alpha-gal syndrome and processed under stringent conditions. Alpha-gal syndrome is an IgE-mediated, type 2 immune (allergic) condition: sensitization to the carbohydrate galactose-alpha-1,3-galactose follows tick bites, driven in the United States by the lone star tick, Amblyomma americanum. These cryopreserved peripheral blood mononuclear cells (PBMCs) support basophil activation testing, allergen-specific T cell work, and effector-cell studies.
Alpha-Gal Immunology and Why PBMC
- IgE to alpha-gal: sensitized donors carry specific IgE against galactose-alpha-1,3-galactose, a glycan present on non-primate mammalian tissue but absent in humans and Old World primates.
- Tick-bite sensitization: bites from Amblyomma americanum in the US, and related ixodid ticks elsewhere, drive class switching to alpha-gal-specific IgE. Repeated bites can boost levels, and sensitization may wane if further bites are avoided.
- Characteristically delayed reactions: symptoms typically begin 3 to 6 hours after eating mammalian meat, because the allergen is carried on chylomicrons and other lipid particles and only reaches the circulation after digestion and lipid processing. This distinguishes alpha-gal syndrome from the immediate onset of most food allergies.
- Effector cells: basophils and mast cells bearing Fc-epsilon-RI-bound alpha-gal-specific IgE are the proximal effectors, which makes PBMCs the correct specimen for basophil activation testing using CD63 and CD203c upregulation on the donor’s own cells.
- Allergen-specific T cells: Th2 responses and the IL-4 and IL-13-driven B cell help behind IgE production can be interrogated by stimulating donor PBMCs with alpha-gal-bearing glycoproteins.
- Clinically important cross-reactivity: alpha-gal IgE underlies hypersensitivity to cetuximab and can affect gelatin-containing products and some bovine or porcine-derived medical materials, making donor cells useful for biologic and device immunogenicity testing.
Donor Stratification Available
- Alpha-gal-specific IgE positive by clinical serology where documented
- Reaction severity: cutaneous-predominant, gastrointestinal-predominant, or a history of anaphylaxis
- Documented tick-bite exposure history and geographic region
- Trigger profile: red meat, organ meat, dairy, or gelatin sensitivity
- Concurrent atopy: asthma, allergic rhinitis, atopic dermatitis, or additional food allergy
- Time since diagnosis and current avoidance status
- Matched healthy controls available, including non-sensitized donors from tick-endemic regions
Product Features
- Research Use Only (RUO), cryopreserved PBMCs
- Clinically confirmed Alpha-Gal Syndrome donors
- Available in cell counts ranging from 5 million to 2 billion
- Processed within 24 hours of collection
- Stored using CryoStor® CS10 freezing media
- IRB-approved protocols and electronic informed consent
- Screen LeukoLot™ available prior to bulk orders
De-identified Donor Data
- Verified Alpha-Gal Syndrome diagnosis
- Donor demographics: age, sex assigned at birth, race/ethnicity
- Self-reported allergies, atopic history, and infectious disease history
- Tick-bite history and reaction profile where documented
- Additional Alpha-Gal Syndrome-specific data available upon request
Applications
- Basophil activation testing with alpha-gal glycoconjugates
- Allergen-specific T cell proliferation and cytokine response assays
- Basophil and mast cell degranulation mechanism studies
- Th2 cytokine and IgE class-switch pathway analysis
- Anti-IgE and IgE-pathway therapeutic screening
- Cetuximab and biologic hypersensitivity research
- Transcriptomic and proteomic profiling of sensitized versus non-sensitized donors
- Diagnostic and assay development for alpha-gal sensitization
Other Alpha-Gal Syndrome Specimen Types
- Alpha-Gal Syndrome Plasma
- Alpha-Gal Syndrome Serum
- Alpha-Gal Syndrome Whole Blood
- Alpha-Gal Syndrome Leukopak
- Alpha-Gal Syndrome Bulk Plasma
Compliance and Quality Assurance
- IRB-approved collections and protocols
- 21 CFR Part 11-compliant e-consent system
- HIPAA-compliant donor data protection
Ordering & Customization
Alpha-Gal Syndrome PBMC samples are shipped on dry ice and available in custom aliquots. For pricing, current availability, international orders, or regulatory requirements, please contact learnmore@sanguinebio.com to confirm documentation and compliance needs. To browse other conditions and live stock status, visit our human PBMC page.
Protocols & Documentation
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PBMC Isolation from Whole Blood
Standard operating procedure for isolating peripheral blood mononuclear cells from whole blood using density-gradient separation.
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Immune Cell Isolation
Guidelines for isolating immune cell populations with validated enrichment steps, purity checkpoints, and documentation.
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Choosing the Right PBMC Configuration
Reference guide comparing PBMC formats, cryopreservation states, and study-fit recommendations for your application.
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Download
Thawing Cryopreserved PBMC
Validated thawing protocol to recover viable cryopreserved PBMCs while minimizing activation and loss of function.
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Informed Consent Form (ICF)
Available upon request — confirms donor consent for research use and downstream commercialization.
PBMC
Frequently Asked Questions
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YES – All collections are conducted under IRB-approved protocols and electronic informed consent. Sanguine utilizes Advarra and WCG IRB for oversight.
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YES – all our products are research use only (RUO).
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You can find our full product catalog here.
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Storage depends on sample type but often cryopreserved samples stored in liquid nitrogen can be stored for years.
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Extensive customization is available. To discuss your project request a quote or email us at learnmore@sanguinebio.com.
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Sanguine offers flexible prospective collection services tailored to fit your research. For more information and to request a quote, please see: our prospective biospecimen collection services page.
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Timelines depend on condition, sample type, and I/E criteria but we often start collection within 2 weeks of signed agreement.
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YES – we have in-stock inventory. You can also email us at learnmore@sanguinebio.com as we continually get new samples in our inventory.
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Each sample from a different individual – Unique donor means each sample comes from a different person, ensuring biological diversity in your study.
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YES – Unique donors can be specified and guaranteed based on your requirements.
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For information about sample quality, please see: Quality and Compliance
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YES – “IRB-approved collection protocols” and IRB approval documentation available upon request. Sanguine utilizes two internationally-recognized IRBs (Advarra and WCG IRB) for review and approval.
Ask a Question
Need a custom PBMC cohort?
Our scientific team can scope prospective collections with donor-specific I/E criteria, typically starting within 2 weeks of agreement.