Atopic Dermatitis PBMC
Powered by BiozAbout:Atopic Dermatitis PBMC — cryopreserved peripheral blood mononuclear cells from IRB-consented donors with clinically confirmed atopic dermatitis, processed within 24 hours and stored in CryoStor CS10. Suited to Th2 and CLA+ skin-homing T cell phenotyping, ex vivo cytokine production assays, STAT6 and JAK-STAT signalling readouts, and single-cell profiling. Donors can be stratified by EASI or SCORAD severity and biologic treatment status.
About Atopic Dermatitis PBMC
Atopic Dermatitis PBMC for Type 2 Immunity and Skin-Homing T Cell Research
Atopic Dermatitis PBMC products are sourced from IRB-consented donors clinically diagnosed with atopic dermatitis and processed under stringent conditions. These cryopreserved peripheral blood mononuclear cells enable functional study of the type 2-skewed immune response that drives atopic dermatitis — an allergic inflammatory skin disease in which barrier dysfunction and Th2 cytokine signalling reinforce one another, rather than an autoantibody-mediated condition.
Why PBMCs for Atopic Dermatitis Research
- Circulating Th2 cells are the cellular source of IL-4, IL-13 and IL-31 in atopic dermatitis. PBMCs allow these populations to be phenotyped directly and, on ex vivo stimulation, to be measured for actual cytokine output rather than inferred from soluble levels.
- Skin-homing T cells expressing cutaneous lymphocyte antigen (CLA) and CCR4 — the receptor for the atopic dermatitis-associated chemokine CCL17/TARC — circulate in blood and can be isolated and characterised from PBMCs without needing a biopsy.
- Dupilumab blocks the shared IL-4 receptor alpha chain, so IL-4Ralpha surface expression and downstream STAT6 phosphorylation in PBMCs constitute a genuine target-engagement readout for the anti-type 2 biologic class.
- JAK inhibitors used in atopic dermatitis act on JAK-STAT signalling common to IL-4, IL-13 and IL-31 receptors, making phospho-flow and signalling assays on patient PBMCs directly relevant to that mechanism.
- Regulatory T cell frequency and function, and the balance between effector and regulatory compartments, are studied in atopic dermatitis and require intact viable cells.
- Basophils and innate lymphoid cell populations relevant to type 2 immunity are present in the mononuclear fraction, supporting activation testing and innate type 2 biology in the same donor sample.
Donor Stratification Available
- Disease severity by EASI, SCORAD or Investigator Global Assessment where recorded
- Total IgE level and blood eosinophil count where available
- Treatment status: topical-only, biologic-naive, on dupilumab or other type 2 biologic, on JAK inhibitor, on systemic immunosuppressant, or in washout
- Atopic comorbidity: asthma, allergic rhinitis, food allergy, or atopic dermatitis alone
- Age group, including paediatric and adult donors
- Disease duration and age at onset where reported
- Matched healthy controls available
Product Features
- Research Use Only (RUO), cryopreserved PBMCs
- Clinically confirmed atopic dermatitis donors
- Available in cell counts ranging from 5 million to 2 billion
- Processed within 24 hours of collection
- Stored using CryoStor® CS10 freezing media
- Viability and recovery values reported per lot
- IRB-approved protocols and electronic informed consent
- Screen LeukoLot™ available prior to bulk orders
De-identified Donor Data
- Verified atopic dermatitis diagnosis
- Donor demographics: age, sex assigned at birth, race/ethnicity
- Self-reported allergies and infectious disease history
- Disease severity and treatment history where available
- Additional atopic dermatitis-specific data available upon request
- Explore our Atopic Dermatitis Donor Network
Applications
- Th2 and effector T cell immunophenotyping by flow or spectral cytometry
- Isolation and characterisation of CLA+ CCR4+ skin-homing T cells
- Ex vivo stimulation assays for IL-4, IL-13 and IL-31 production
- STAT6 and JAK-STAT phospho-signalling readouts for biologic and small molecule target engagement
- Regulatory T cell frequency and suppressive function studies
- Basophil and innate type 2 cell activation testing
- Single-cell RNA sequencing and multi-omic profiling of the circulating immune compartment
- Drug screening and therapeutic development in type 2 inflammatory disease
Other Atopic Dermatitis Specimen Types
- Atopic Dermatitis Leukopak
- Atopic Dermatitis Plasma
- Atopic Dermatitis Serum
- Atopic Dermatitis Whole Blood
- Atopic Dermatitis Skin Punch Biopsy
- Atopic Dermatitis CD4+ T Cells
Compliance and Quality Assurance
- IRB-approved collections and protocols
- 21 CFR Part 11 – compliant e-consent system
- HIPAA-compliant donor data protection
Ordering & Customization
Atopic Dermatitis PBMC samples are shipped on dry ice and available in custom aliquots. For pricing, availability, or to discuss a severity-stratified cohort, contact learnmore@sanguinebio.com. For international orders or regulatory requirements, please contact us to confirm documentation and compliance needs. To browse PBMCs across all disease states and healthy donors, visit our human PBMC page.
Donor Metadata
- Verified diagnosis and clinical history
- Demographic data: age, sex assigned at birth, race/ethnicity
- Medication and treatment background when available
Compliance and Quality Assurance
- IRB-approved collections and standardized procedures
- 21 CFR Part 11-compliant e-consent system
- HIPAA-compliant data management
Ordering & Customization
Human PBMC are available in customizable aliquot sizes. For international orders or documentation needs, please contact learnmore@sanguinebio.com to confirm requirements and availability. We also work with multiple ordering platforms and distributors, learn more here.
Protocols & Documentation
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PBMC Isolation from Whole Blood
Standard operating procedure for isolating peripheral blood mononuclear cells from whole blood using density-gradient separation.
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Immune Cell Isolation
Guidelines for isolating immune cell populations with validated enrichment steps, purity checkpoints, and documentation.
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Choosing the Right PBMC Configuration
Reference guide comparing PBMC formats, cryopreservation states, and study-fit recommendations for your application.
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Thawing Cryopreserved PBMC
Validated thawing protocol to recover viable cryopreserved PBMCs while minimizing activation and loss of function.
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Informed Consent Form (ICF)
Available upon request — confirms donor consent for research use and downstream commercialization.
PBMC
Where can I find your complete catalog?
You can find our full product catalog here.
How long can samples be stored?
Storage depends on sample type but often cryopreserved samples stored in liquid nitrogen can be stored for years.
What customization options are available?
Extensive customization is available. To discuss your project request a quote or email us at learnmore@sanguinebio.com.
Do you offer prospective collections?
Sanguine offers flexible prospective collection services tailored to fit your research. For more information and to request a quote, please see: our prospective biospecimen collection services page.
What is the turnaround time for custom collections?
Timelines depend on condition, sample type, and I/E criteria but we often start collection within 2 weeks of signed agreement.
Do you have samples in stock?
YES - we have in-stock inventory. You can also email us at learnmore@sanguinebio.com as we continually get new samples in our inventory.
What does unique donor mean?
Each sample from a different individual - Unique donor means each sample comes from a different person, ensuring biological diversity in your study.
Are samples collected under IRB-approved protocols?
YES - "IRB-approved collection protocols" and IRB approval documentation available upon request. Sanguine utilizes two internationally-recognized IRBs (Advarra and WCG IRB) for review and approval.
Frequently Asked Questions
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804 In Stock View InventoryNeed a custom PBMC cohort?
Our scientific team can scope prospective collections with donor-specific I/E criteria, typically starting within 2 weeks of agreement.