Chronic Myeloid Leukemia (CML) Bone Marrow Mononuclear Cells

Chronic Myeloid Leukemia (CML) Bone Marrow Mononuclear Cells for Leukemia Research

Our Chronic Myeloid Leukemia (CML) Bone Marrow Mononuclear Cells is a hematopoietic tissue sample isolated by density-gradient separation from bone marrow aspirate, capturing the full spectrum of marrow-resident hematopoietic stem, progenitor, and precursor cells that peripheral blood samples do not contain. Samples are sourced from IRB-consented donors clinically diagnosed with CML, a myeloproliferative neoplasm driven by the BCR-ABL1 fusion gene.

CML Biology and Why Bone Marrow

• CML arises from the BCR-ABL1 fusion oncogene originating in a pluripotent hematopoietic stem cell within the bone marrow, giving rise to unchecked myeloid proliferation.
• Marrow cytogenetic analysis is central to diagnosis (identifying the Philadelphia chromosome) and to detecting additional chromosomal abnormalities associated with clonal evolution and disease progression to accelerated or blast phase.
• While peripheral blood BCR-ABL1 transcript levels by qPCR are used for routine monitoring, marrow assessment remains important at diagnosis and at defined treatment milestones, including evaluation for resistance mutations.
• Tyrosine kinase inhibitor (TKI) therapy has transformed CML management, and marrow-derived material supports research into treatment-free remission, resistance mechanisms, and residual leukemic stem cell persistence.
• Leukemic stem cells in CML are relatively TKI-resistant and reside in the marrow niche, making marrow material particularly relevant to relapse and cure research.

Donor Stratification Available

• Disease phase: chronic, accelerated, or blast phase
• Philadelphia chromosome and additional cytogenetic abnormalities where documented
• TKI treatment history and molecular response status
• Resistance mutation status where documented
• Age band and sex assigned at birth
• Matched healthy bone marrow controls available

Product Features

• Isolated from clinically confirmed CML donor bone marrow aspirate
• Density-gradient purified and cryopreserved
• Research Use Only (RUO)
• IRB-approved protocols with documented consent
• Standard and custom cell quantities available

De-identified Donor Data

• CML diagnosis confirmation and disease phase where documented
• Age, sex assigned at birth, and ethnicity
• Donor-reported allergy and infectious disease history
• TKI treatment history where documented
• Additional CML-specific metadata available on request

Applications

• BCR-ABL1 and clonal evolution cytogenetic studies
• TKI resistance mechanism and leukemic stem cell persistence research
• Treatment-free remission biomarker studies
• Marrow niche and leukemic stem cell microenvironment research
• Molecular response and residual disease assay development

Other Specimen Types

For matched marrow-derived material across conditions, see our human bone marrow mononuclear cells collection, and our CML PBMCs for peripheral blood-based studies.

Compliance and Quality Assurance

• IRB-approved and HIPAA-compliant protocols
• Electronic informed consent with 21 CFR Part 11-compliant e-signatures
• Documentation support available for regulatory review

Ordering & Customization

CML bone marrow mononuclear cells are available in standard and custom cell quantities. For pricing, international orders, or documentation requirements, please email learnmore@sanguinebio.com to ensure compatibility with your country’s regulations.