Hepatitis B Plasma
Hepatitis B Plasma — human plasma from IRB-consented donors with confirmed HBV infection, stratifiable by HBeAg status, viral load, and treatment. Ideal for HBV DNA and quantitative HBsAg assay work, antiviral response research, and biomarker discovery.
Hepatitis B Plasma for Virology and Infectious Disease Research
Hepatitis B Plasma is collected from IRB-consented donors clinically diagnosed with hepatitis B virus (HBV) infection and processed within hours of collection to preserve viral nucleic acid, circulating viral antigens, cytokines, and metabolites. Custom aliquot volumes and anticoagulant types are available.
Why Plasma for Hepatitis B Research
HBV is a chronic viral infection, and the research question is the interaction between a persistent hepatotropic virus and the host immune response — not autoimmunity. Plasma is the standard matrix for HBV nucleic acid and antigen quantification, because it is collected with anticoagulant and separated without a clotting step, avoiding the analyte changes and cellular release that clotting introduces.
- Circulating viral markers in one matrix — HBV DNA, hepatitis B surface antigen (HBsAg), and hepatitis B e antigen (HBeAg) are all measurable in plasma, allowing viral load and antigen burden to be assessed together
- HBsAg is produced in vast excess — subviral particles greatly outnumber infectious virions, which is why quantitative HBsAg is a distinct readout from HBV DNA and why HBsAg loss defines functional cure
- Persistence is nuclear, not circulatory — covalently closed circular DNA (cccDNA) persists in hepatocyte nuclei and is not eliminated by nucleoside analogue therapy, so plasma markers are the practical non-invasive window on residual viral activity
- Disease phase drives interpretation — HBeAg-positive and HBeAg-negative chronic infection and chronic hepatitis differ in viral load, ALT, and immune activity; specifying phase is essential to cohort design
- Exhausted virus-specific T cell response — the functional impairment of HBV-specific T cells in chronic infection is reflected in circulating cytokine and chemokine profiles measurable in plasma
- Anticoagulant choice matters — EDTA plasma is the conventional matrix for viral nucleic acid quantification; alternative anticoagulants are available where downstream assays require them
Donor Stratification Available
- HBeAg status — HBeAg-positive versus HBeAg-negative infection
- Viral load and antigen level — HBV DNA and quantitative HBsAg reported per lot where measured
- Treatment status — untreated, on nucleoside/nucleotide analogue therapy, or previously treated
- Liver status — ALT level, fibrosis stage, or cirrhosis where recorded
- Co-infection — hepatitis D, hepatitis C, or HIV co-infection where documented; genotype where available
- Matched healthy controls available — demographically matched HBV-negative donors
Product Features
- Research Use Only (RUO)
- Plasma collected from clinically confirmed Hepatitis B donors
- Processed within one day of collection
- IRB-approved protocols and informed consent
- Custom aliquot volumes and anticoagulant types available upon request
De-identified Donor Data
- Verified Hepatitis B diagnosis
- Demographic data: age, sex assigned at birth, race/ethnicity
- Donor-reported medications, allergies, and comorbidities
- Additional Hepatitis B-specific metadata, including serological and virological values, available on request
Applications
- HBV DNA quantification and viral kinetics research
- Quantitative HBsAg and HBeAg assay development and validation
- Serological assay and diagnostic panel development
- Antiviral and functional-cure candidate response research
- Cytokine, chemokine, and inflammation profiling in chronic infection
- Liver fibrosis and disease progression biomarker discovery
- Proteomic and metabolomic profiling across infection phases
- Reference and control material for infectious disease testing platforms
Other Hepatitis B Specimen Types
Matched material from the same HBV donor pool is available as hepatitis B serum, whole blood, PBMC, and leukopak. Larger volumes are available as hepatitis B bulk plasma.
Compliance and Quality Assurance
- IRB-approved and HIPAA-compliant protocols
- Electronic informed consent with 21 CFR Part 11 – compliant e-signatures
- Documentation support available for regulatory review
Ordering & Customization
Hepatitis B plasma is available in standard and custom volumes. For pricing, international orders, or documentation requirements, please email learnmore@sanguinebio.com to ensure compatibility with your country’s regulations. To browse the full specimen range and healthy-donor controls, see our human plasma collection.
Protocols & Documentation
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Download
Plasma Isolation
Protocol for plasma separation from anticoagulated whole blood, including processing, storage, and traceability requirements.
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Informed Consent Form (ICF)
Available upon request — confirms donor consent for research use and downstream commercialization.
Plasma
Frequently Asked Questions
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YES – All collections are conducted under IRB-approved protocols and electronic informed consent. Sanguine utilizes Advarra and WCG IRB for oversight.
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YES – all our products are research use only (RUO).
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You can find our full product catalog here.
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Storage depends on sample type but often cryopreserved samples stored in liquid nitrogen can be stored for years.
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Extensive customization is available. To discuss your project request a quote or email us at learnmore@sanguinebio.com.
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Sanguine offers flexible prospective collection services tailored to fit your research. For more information and to request a quote, please see: our prospective biospecimen collection services page.
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Timelines depend on condition, sample type, and I/E criteria but we often start collection within 2 weeks of signed agreement.
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YES – we have in-stock inventory. You can also email us at learnmore@sanguinebio.com as we continually get new samples in our inventory.
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Each sample from a different individual – Unique donor means each sample comes from a different person, ensuring biological diversity in your study.
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YES – Unique donors can be specified and guaranteed based on your requirements.
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For information about sample quality, please see: Quality and Compliance
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YES – “IRB-approved collection protocols” and IRB approval documentation available upon request. Sanguine utilizes two internationally-recognized IRBs (Advarra and WCG IRB) for review and approval.
Ask a Question
Need a custom Plasma cohort?
Our scientific team can scope prospective collections with donor-specific I/E criteria, typically starting within 2 weeks of agreement.