Hypertension PBMC
Hypertension PBMC samples are isolated from IRB-consented donors with clinically diagnosed hypertension. Supplied for genotyping, transcriptomic and epigenomic profiling, and research into the immune contribution to vascular inflammation, with matched normotensive controls available.
Hypertension PBMC for Cardiovascular Genomics and Vascular Inflammation Research
Hypertension PBMC samples are collected from IRB-consented donors clinically diagnosed with hypertension (high blood pressure) and processed under controlled conditions within 24 hours of collection. Hypertension itself is a vascular and metabolic disorder — driven by the renin-angiotensin-aldosterone system, sympathetic tone, renal sodium handling, endothelial dysfunction, and vascular remodelling — so these cryopreserved peripheral blood mononuclear cells are supplied for genomic, transcriptomic, and immune-mechanism work rather than as a primary diagnostic assay matrix for blood pressure.
What These Cells Are Used For
- Genotyping and genetic association work: PBMCs are a convenient, high-quality source of genomic DNA for candidate-gene and genome-wide studies of blood pressure regulation, including RAAS pathway and sodium-handling loci.
- Transcriptomics: bulk and single-cell RNA sequencing of circulating leukocytes from hypertensive donors, including comparisons across treatment status and comorbidity.
- Immune contribution to vascular inflammation: the role of T cells, monocytes, and macrophages in vascular and perivascular inflammation is an active area of experimental hypertension research; these donors support translational human work in that area. This is a research hypothesis under investigation, not established clinical diagnostics.
- Monocyte and T-cell phenotyping by flow cytometry, mass cytometry, or CITE-seq, with paired de-identified clinical metadata.
- Ex vivo stimulation and functional assays to compare leukocyte responses between hypertensive donors and matched normotensive controls.
- Epigenetics: DNA methylation and chromatin accessibility profiling in cardiometabolic cohorts.
Donor Stratification Available
- Essential (primary) versus secondary hypertension
- Treated versus untreated / treatment-naive donors
- Antihypertensive drug class: ACE inhibitor, angiotensin receptor blocker, thiazide or thiazide-like diuretic, calcium channel blocker, beta blocker, mineralocorticoid receptor antagonist, or combination therapy
- Resistant hypertension
- Comorbid chronic kidney disease, type 2 diabetes, obesity, or dyslipidaemia
- Established target-organ damage, including left ventricular hypertrophy, prior stroke, or coronary artery disease
- Matched healthy normotensive controls available
Product Features
- Research Use Only (RUO), cryopreserved hypertension PBMC samples
- Clinically confirmed hypertension donors
- Available in cell counts ranging from 5 million to 2 billion
- Processed within 24 hours of collection
- Preserved using CryoStor® CS10 freezing media
- IRB-approved protocols and electronic informed consent
- Screening (LeukoLot™) available prior to bulk orders
De-identified Donor Data
- Verified hypertension diagnosis
- Donor demographics: age, sex assigned at birth, race/ethnicity
- Current medications, including antihypertensive class
- Self-reported allergies and infectious disease history
- Additional hypertension-specific clinical data available on request
Applications
- Genomic DNA extraction and genotyping for blood pressure and cardiometabolic loci
- Bulk and single-cell transcriptomic profiling of circulating leukocytes
- Immunophenotyping of monocyte and T-cell subsets in hypertensive versus normotensive donors
- Research into the immune contribution to vascular and perivascular inflammation
- Epigenomic profiling, including DNA methylation and ATAC-seq
- Ex vivo stimulation and cytokine release assays
- Drug screening and target validation in cardiovascular programmes
- Proteomic profiling of leukocyte lysates
Other Hypertension Specimen Types
Hypertension donor material is also available as Hypertension Serum, Hypertension Plasma, Hypertension Whole Blood, and Hypertension Leukopak. For serum-based renin, aldosterone, and natriuretic peptide measurements, the serum product is the appropriate matrix.
Compliance and Quality Assurance
- IRB-approved collections and protocols
- 21 CFR Part 11-compliant e-consent
- HIPAA-compliant donor data protection
Ordering & Customization
Hypertension PBMC samples ship on dry ice in standard or custom aliquots. For pricing, international orders, or regulatory documentation, contact learnmore@sanguinebio.com. Browse all human PBMCs or request a custom disease-state collection.
Protocols & Documentation
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Download
PBMC Isolation from Whole Blood
Standard operating procedure for isolating peripheral blood mononuclear cells from whole blood using density-gradient separation.
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Download
Immune Cell Isolation
Guidelines for isolating immune cell populations with validated enrichment steps, purity checkpoints, and documentation.
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Download
Choosing the Right PBMC Configuration
Reference guide comparing PBMC formats, cryopreservation states, and study-fit recommendations for your application.
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Download
Thawing Cryopreserved PBMC
Validated thawing protocol to recover viable cryopreserved PBMCs while minimizing activation and loss of function.
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Informed Consent Form (ICF)
Available upon request — confirms donor consent for research use and downstream commercialization.
PBMC
Frequently Asked Questions
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YES – All collections are conducted under IRB-approved protocols and electronic informed consent. Sanguine utilizes Advarra and WCG IRB for oversight.
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YES – all our products are research use only (RUO).
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You can find our full product catalog here.
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Storage depends on sample type but often cryopreserved samples stored in liquid nitrogen can be stored for years.
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Extensive customization is available. To discuss your project request a quote or email us at learnmore@sanguinebio.com.
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Sanguine offers flexible prospective collection services tailored to fit your research. For more information and to request a quote, please see: our prospective biospecimen collection services page.
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Timelines depend on condition, sample type, and I/E criteria but we often start collection within 2 weeks of signed agreement.
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YES – we have in-stock inventory. You can also email us at learnmore@sanguinebio.com as we continually get new samples in our inventory.
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Each sample from a different individual – Unique donor means each sample comes from a different person, ensuring biological diversity in your study.
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YES – Unique donors can be specified and guaranteed based on your requirements.
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For information about sample quality, please see: Quality and Compliance
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YES – “IRB-approved collection protocols” and IRB approval documentation available upon request. Sanguine utilizes two internationally-recognized IRBs (Advarra and WCG IRB) for review and approval.
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Need a custom PBMC cohort?
Our scientific team can scope prospective collections with donor-specific I/E criteria, typically starting within 2 weeks of agreement.