Allergy Leukopak

About Allergy Leukopak

Allergy Leukopak for Type 2 Immunity and Allergic Disease Research

LeukoCore Allergy Leukopak biospecimens are collected from IRB-consented donors clinically diagnosed with allergy through IRB-approved apheresis procedures. Each leukopak contains large quantities of peripheral blood mononuclear cells, is processed and shipped within 24 hours of collection, and is available fresh or cryopreserved. Research Use Only (RUO).

Allergic Immunology and Why a Leukopak

• Allergic disease is a type 2 immune response, not an autoimmune one: sensitization to an environmental or food allergen drives class switching to allergen-specific IgE rather than autoantibody production.
• Allergen-specific IgE binds the high-affinity receptor FcεRI on mast cells and basophils; re-exposure crosslinks receptor-bound IgE and triggers immediate mediator release.
• The adaptive arm is dominated by Th2-polarized CD4 T cells producing IL-4, IL-5, and IL-13, supported by epithelial alarmins and group 2 innate lymphoid cells; IL-5 drives eosinophil recruitment and survival.
• These are the pathways targeted by approved biologics against IgE, IL-4 receptor alpha, and IL-5, so functional immune cells are central to mechanism and screening work.
• A leukopak delivers far more cells per donor than a standard blood draw, which is what makes basophil activation testing, allergen-specific T cell expansion, and multi-assay panels from a single donor practical.
• High per-donor yield also enables repeat sampling designs and reduces donor-to-donor variability across large screening campaigns.

Donor Stratification Available

• Allergen class: environmental or aeroallergen, food, drug, or insect venom
• Specific sensitization where documented, including pollen, dust mite, animal dander, or named food allergens
• Clinical severity and history of anaphylaxis
• Atopic comorbidities: asthma, atopic dermatitis, allergic rhinitis
• Treatment status: allergen immunotherapy, biologic therapy, antihistamine only, or untreated
• Age, sex, and self-reported race/ethnicity
• Matched healthy controls available

Product Features

• Research Use Only (RUO) human leukopaks
• Clinically confirmed allergy donors
• High cell yield for large-scale studies
• Processed and shipped within 24 hours of collection
• Fresh or cryopreserved options available
• IRB-approved and ethically sourced
• Infectious disease testing
• HLA-A typing
• Screen donors with LeukoLot™

De-identified Donor Data

• Verified allergy diagnosis from healthcare providers
• Donor demographics: age, sex assigned at birth, race/ethnicity
• Self-reported medication history and allergies
• Relevant clinical data including allergy severity and allergen class where available

Applications

• Basophil activation and mediator release assays
• Allergen-specific CD4 T cell expansion and Th2 cytokine profiling
• Immunophenotyping of type 2 immune compartments by flow or spectral cytometry
• Ex vivo allergen and adjuvant stimulation studies
• Screening and mechanism-of-action work for anti-IgE and type 2 cytokine-directed candidates
• Cell isolation for downstream monocyte, dendritic cell, or B cell studies
• Single-cell and bulk transcriptomic and multi-omic profiling
• Allergen immunotherapy response and tolerance-induction research

Other Allergy Specimen Types

• Allergy PBMC
• Allergy Bulk Plasma
• Peanut Allergy Leukopak

Compliance and Quality Assurance

• Collected under IRB oversight with donor e-consent (21 CFR Part 11 compliant)
• HIPAA-compliant handling of donor information
• Rigorous donor screening and documentation

Ordering & Customization

Allergy Leukopak samples are available fresh or cryopreserved. For pricing, current availability, international shipping, or regulatory documentation, please contact learnmore@sanguinebio.com to confirm regional compliance and shipping availability. To browse other disease-state options and live stock status, visit our human leukopak page.