Alopecia PBMC

About Alopecia PBMC

Alopecia PBMC for Autoimmune Hair Loss Research

Alopecia PBMC samples are cryopreserved peripheral blood mononuclear cells isolated from IRB-consented donors with a clinically confirmed diagnosis of alopecia. Cells are processed within 24 hours of collection and cryopreserved in CryoStor® CS10. The cohort is built primarily around alopecia areata, the immune-mediated form of hair loss and the subtype most often studied in translational dermatology and immunology programmes; other subtypes are available and are annotated at the donor level.

Why Subtype Matters: The Immunobiology of Alopecia Areata

“Alopecia” is a descriptive term rather than a single disease, and the mechanisms behind its subtypes are genuinely different. Peripheral blood is most informative for the immune-mediated forms:

• Alopecia areata is autoimmune. Collapse of hair-follicle immune privilege permits CD8+ T cells, including an NKG2D-expressing cytotoxic population, to attack the anagen follicle, producing the characteristic peribulbar lymphocytic infiltrate
• Interferon-gamma and IL-15 signalling sustain that attack through the JAK-STAT pathway — the mechanistic basis for the clinical activity of JAK inhibitors in alopecia areata
• Genetic risk maps to HLA class II and to loci shared with other autoimmune conditions; alopecia areata frequently co-occurs with thyroid autoimmunity and vitiligo
• Androgenetic alopecia is not autoimmune — it is progressive follicular miniaturisation driven by androgen signalling on a polygenic background, and should not be pooled with alopecia areata in immune studies
• Why PBMC: circulating T-cell subsets, NK and NK-like populations, and cytokine responses can be immunophenotyped, sorted, stimulated, and sequenced from the same donor material
• The cryopreserved format supports batched, longitudinal, or multi-site experiments on one defined donor set

Donor Stratification Available

• Subtype: alopecia areata, alopecia totalis, alopecia universalis, or androgenetic alopecia
• Extent and pattern of hair loss, including scalp-limited versus body involvement
• Disease duration and relapsing versus stable or chronic course
• Treatment status: treatment-naive, topical or intralesional corticosteroids, or JAK inhibitor therapy
• Coexisting autoimmune diagnoses such as thyroid disease or vitiligo
• Age, sex assigned at birth, and race/ethnicity
• Matched healthy controls available

Product Features

• Research Use Only (RUO), cryopreserved PBMCs
• Clinically confirmed alopecia donors, with subtype annotated where documented
• Available in cell counts ranging from 5 million to 2 billion
• Processed within 24 hours of collection
• Stored using CryoStor® CS10 freezing media
• IRB-approved protocols and electronic informed consent
• Screen LeukoLot™ available prior to bulk orders

De-identified Donor Data

• Verified alopecia diagnosis, with subtype where documented
• Donor demographics: age, sex assigned at birth, race/ethnicity
• Self-reported allergies and infectious disease history
• Medication history and additional alopecia-specific clinical data available upon request

Applications

• Immunophenotyping of CD8+ and CD4+ T-cell subsets in alopecia areata
• Characterisation of NK and NKG2D+ cytotoxic populations
• JAK-STAT pathway signalling and JAK-inhibitor mechanism-of-action studies
• Interferon-gamma and IL-15 stimulation and cytokine release assays
• Bulk and single-cell RNA sequencing of circulating immune cells
• T-cell receptor repertoire analysis
• Biomarker discovery for disease activity and treatment response
• Comparative studies across alopecia subtypes and against matched healthy controls

Other Alopecia Specimen Types

• Alopecia Leukopak — apheresis-derived, for high cell-number requirements
• Alopecia Whole Blood — fresh, for same-day cellular assays and germline genotyping
• Alopecia Plasma and Alopecia Serum — for circulating cytokine and protein work
• Alopecia Bulk Plasma — larger volumes for assay development
• Alopecia Skin Punch Biopsy — lesional tissue for follicular histology and infiltrate analysis

Compliance and Quality Assurance

• IRB-approved collections and protocols
• 21 CFR Part 11-compliant e-consent system
• HIPAA-compliant donor data protection

Ordering & Customization

Alopecia PBMC samples are shipped on dry ice and available in custom aliquots. For pricing, international orders, or regulatory requirements, please contact learnmore@sanguinebio.com to confirm documentation and compliance needs. To browse healthy-donor controls and the full specimen range, see our human PBMCs collection.