Alzheimer’s Disease PBMC

Alzheimer’s Disease PBMC for Neurodegeneration Research

Alzheimer’s Disease PBMC products are cryopreserved peripheral blood mononuclear cells from IRB-consented donors with a clinically confirmed diagnosis of Alzheimer’s disease. Cells are processed within 24 hours of collection and cryopreserved in CryoStor® CS10 to support neurodegeneration research, host genetics, and peripheral immune studies.

Alzheimer’s Disease Biology and What Peripheral Cells Can Tell You

Alzheimer’s disease is a progressive neurodegenerative disorder, not an autoimmune one. Its defining pathology is extracellular amyloid-beta plaque deposition and intraneuronal neurofibrillary tangles of hyperphosphorylated tau, with synaptic loss, neuroinflammation, and regional atrophy. We are deliberately clear about what a blood-derived cell product can and cannot address:

• Genotyping. APOE is the strongest common genetic risk factor for late-onset disease, and PBMCs are a convenient source of genomic DNA for APOE and polygenic risk work
• Transcriptomics and epigenetics. Bulk and single-cell RNA sequencing of circulating immune cells is used to look for peripheral signatures that track with disease status
• Peripheral immune contribution. Monocyte and T-cell phenotype and function are of active interest given the role of microglia and innate immune genes such as TREM2 in disease risk
• iPSC derivation. PBMCs are a standard starting material for reprogramming into induced pluripotent stem cells and differentiation into neurons, astrocytes, or microglia-like cells from a genotyped donor
• Honest limitation: PBMCs do not report central nervous system pathology directly. Fluid biomarkers of amyloid and tau pathology — amyloid-beta, phosphorylated tau (p-tau181, p-tau217), neurofilament light, and GFAP — are measured in the matched plasma, serum, and cerebrospinal fluid products linked below
• Paired specimens from the same donor allow genotype and cellular phenotype to be read alongside those fluid biomarkers

Donor Stratification Available

• Disease stage: mild cognitive impairment, mild, moderate, or severe Alzheimer’s disease
• Age at onset, including early-onset versus late-onset presentation
• APOE genotype where available
• Family history of dementia
• Treatment status, including cholinesterase inhibitors, memantine, or anti-amyloid antibody therapy
• Age, sex assigned at birth, and race/ethnicity
• Matched, age-matched healthy controls available

Product Features

• Research Use Only (RUO), cryopreserved PBMCs
• Clinically confirmed Alzheimer’s disease donors
• Processed within 24 hours of collection
• Stored using CryoStor® CS10 freezing media
• IRB-approved protocols and electronic informed consent
• Paired plasma, serum, whole blood, or cerebrospinal fluid available from the same donor
• Screen LeukoLot™ available prior to bulk orders

De-identified Donor Data

• Verified Alzheimer’s disease diagnosis
• Donor demographics: age, sex assigned at birth, race/ethnicity
• Self-reported allergies and infectious disease history
• Cognitive assessment results, medication history, and additional Alzheimer’s disease-specific data available upon request

Applications

• APOE genotyping and polygenic risk score research
• Bulk and single-cell transcriptomic profiling of circulating immune cells
• Peripheral monocyte and T-cell immunophenotyping in neurodegeneration
• iPSC reprogramming and derivation of neurons, astrocytes, and microglia-like cells
• DNA methylation and epigenetic ageing studies
• Drug screening and target validation in patient-derived cells
• Multi-omic integration with matched fluid biomarker data
• Case-control comparison against age-matched cognitively normal donors

Other Alzheimer’s Disease Specimen Types

• Alzheimer’s Disease Plasma — for p-tau, amyloid-beta, NfL, and GFAP immunoassay work
• Alzheimer’s Disease Serum — for serum protein biomarker panels
• Alzheimer’s Disease Whole Blood — fresh, for germline genotyping and same-day cellular assays
• Alzheimer’s Disease Cerebrospinal Fluid — the compartment closest to CNS pathology

Compliance and Quality Assurance

• IRB-approved collections and protocols
• 21 CFR Part 11-compliant e-consent system
• HIPAA-compliant donor data protection

Ordering & Customization

Alzheimer’s Disease PBMC samples are shipped on dry ice and available in custom aliquots. For pricing, international orders, or regulatory requirements, please contact learnmore@sanguinebio.com to confirm documentation and compliance needs. For healthy-donor controls and the full specimen range, see our human PBMCs collection.