Celiac Disease CD3+ T Cells

About Celiac Disease CD3+ T Cells

Celiac Disease CD3+ T Cells for Autoimmune and Immunopathology Research

Celiac Disease CD3+ T Cells are isolated from the peripheral blood of IRB-consented adult donors diagnosed with celiac disease, processed within 24 hours of collection and cryopreserved in CryoStor CS10. These enriched CD3+ T lymphocytes support antigen-specific T cell work in one of the best-characterised human autoimmune diseases.

Celiac Disease T Cell Biology and Why CD3+ Cells

Celiac disease is an autoimmune enteropathy triggered by dietary gluten in genetically susceptible individuals, and it is unusual in that both the HLA restriction and the driving antigen are known.

• Nearly all patients carry HLA-DQ2 or HLA-DQ8, which present deamidated gluten peptides to CD4+ T cells; HLA typing is effectively a prerequisite for antigen-specific experiments
• Tissue transglutaminase deamidates glutamine residues in gluten peptides, increasing their affinity for DQ2 and DQ8 and creating the immunodominant epitopes
• Gluten-specific CD4+ T cells provide help that drives autoantibody production against tissue transglutaminase, the basis of standard anti-tTG serology
• Intraepithelial cytotoxic lymphocytes mediate the villous atrophy and crypt hyperplasia seen on duodenal biopsy, with IL-15 implicated in their activation
• Gluten-specific CD4+ T cells are detectable in peripheral blood, particularly after gluten challenge, which is why a CD3-enriched peripheral product is a practical alternative to biopsy-derived cells
• CD3+ enrichment removes monocytes and B cells that confound T cell readouts, giving cleaner tetramer staining, proliferation assays and TCR repertoire sequencing

Donor Stratification Available

• HLA-DQ2 versus HLA-DQ8 genotype where typed
• Anti-tissue transglutaminase antibody status and titre where available
• Diagnostic confirmation by serology alone or by duodenal biopsy
• Dietary status: gluten-free diet adherent, non-adherent, or newly diagnosed and untreated
• Presence of comorbid autoimmune disease, such as type 1 diabetes or autoimmune thyroid disease
• Matched healthy controls available, including HLA-matched non-celiac donors on request

Product Features

• Research Use Only (RUO), CD3+ enriched T cells
• Isolated from clinically confirmed Celiac Disease donors
• High viability and CD3+ cell enrichment
• Cryopreserved in CryoStor® CS10 media
• Processed within 24 hours of collection
• IRB-approved collection and HIPAA-compliant handling
• Standard or custom aliquot sizes available

De-identified Donor Data

• Confirmed Celiac Disease diagnosis
• Demographics: age, sex assigned at birth, race/ethnicity
• Clinical history and medication records available
• HLA and serology data where available

Applications

• Gluten-specific CD4+ T cell detection, including tetramer-based approaches
• T cell subset analysis and immune profiling in celiac disease
• TCR repertoire sequencing and clonality analysis
• Antigen-specific proliferation and cytokine production assays
• Exhaustion, activation and memory phenotype characterisation
• Target validation and preclinical drug development for gluten-directed therapeutics
• Tolerance induction and immune modulation modelling
• Comparative studies against matched healthy control T cells

Other Celiac Disease Specimen Types

• Celiac Disease CD4+ T Cells
• Celiac Disease CD8+ T Cells
• Celiac Disease CD19+ B Cells
• Celiac Disease CD56+ NK Cells
• Celiac Disease PBMC
• Celiac Disease Leukopak
• Celiac Disease Serum

Compliance and Quality Assurance

• IRB-approved protocols and 21 CFR Part 11-compliant e-consent
• Standardized isolation and cryopreservation
• HIPAA-compliant data protection and donor confidentiality

Ordering & Customization

Celiac Disease CD3+ T Cells are available in standard or custom aliquot sizes. For pricing, availability, international shipments or documentation, contact learnmore@sanguinebio.com. To browse CD3+ T cells from other disease states and check live stock status, visit our human CD3+ T cells page.