Peanut Allergy PBMC

Peanut Allergy PBMC for Type 2 Immunity and Food Allergy Research

Peanut Allergy Human PBMC products are sourced from IRB-consented donors clinically diagnosed with peanut allergy, processed within 24 hours of collection and cryopreserved in CryoStor CS10 freezing media. Peanut allergy is an IgE-mediated type 2 allergic disease, driven by allergen-specific Th2 responses rather than by autoimmunity, and PBMC are the specimen that makes the cellular arm of that response experimentally accessible.

Why PBMC for Peanut Allergy Research

• Allergen-specific T-cell responses cannot be measured in serum. They require live antigen-presenting cells and T cells together in the same well, which is precisely what a PBMC preparation provides: peanut extract or peptide pools can be added directly to autologous APC-plus-T-cell cultures.
• The pathogenic axis runs through Th2 cells producing IL-4, IL-5 and IL-13, which drive IgE class switching and arm effector cells. PBMC support ex vivo stimulation with cytokine readout by ELISA, multiplex, ELISpot or intracellular staining in the disease-relevant cell population.
• Allergen-specific CD4 T cells are rare in circulation, so their detection depends on enrichment or activation-induced marker approaches such as CD154 upregulation, or on MHC class II tetramers. Both require substantial viable PBMC input, and the pathogenic CD4 subset described in food allergy carries a distinctive terminally differentiated Th2 phenotype identifiable by surface markers.
• Basophils are present at low frequency within PBMC preparations, which allows basophil activation testing and FcepsilonRI cross-linking studies on autologous cells rather than on passively sensitised donor cells.
• Sensitisation in peanut allergy is component-resolved: reactivity to the 2S albumin Ara h 2 is the most predictive of clinical reactivity, while Ara h 8 reactivity often reflects birch pollen cross-reactivity in donors who tolerate peanut. Stimulating PBMC with individual components rather than crude extract separates genuine effector responses from cross-reactive ones.
• Oral immunotherapy and anti-IgE therapy have changed the treatment landscape, and both act on mechanisms best interrogated in cells: regulatory T-cell induction, Th2 anergy and effector-cell desensitisation. Cryopreserved PBMC from donors at different treatment stages support that mechanistic work.

Donor Stratification Available

• Diagnostic basis: oral food challenge-proven reactivity versus sensitisation established by specific IgE or skin prick test
• Peanut-specific IgE level and component-resolved results where documented, including Ara h 2 specific IgE, plus skin prick test wheal size
• Reaction severity and history of anaphylaxis or epinephrine use
• Treatment status: immunotherapy-naive, on oral immunotherapy, post-desensitisation maintenance, or receiving anti-IgE or type 2 targeted biologic therapy
• Atopic comorbidities: asthma, atopic dermatitis, allergic rhinitis, eosinophilic oesophagitis, and other food allergies including tree nut
• Age group, including paediatric and adult donors where available, with matched non-allergic healthy controls

Product Features

• Research Use Only (RUO), cryopreserved PBMCs
• Clinically confirmed peanut allergy donors
• Available in cell counts ranging from 5 million to 2 billion
• Processed within 24 hours of collection
• Stored using CryoStor CS10 freezing media
• Viability and cell counts reported per lot
• IRB-approved protocols and electronic informed consent
• Screen LeukoLot available prior to bulk orders

De-identified Donor Data

• Verified peanut allergy diagnosis
• Donor demographics: age, sex assigned at birth, race/ethnicity
• Self-reported allergies and infectious disease history
• Additional peanut allergy-specific data, including specific IgE and reaction history, available upon request

Applications

• Allergen-specific T-cell stimulation with peanut extract or Ara h component peptides
• Th2 cytokine profiling by ELISpot, multiplex or intracellular cytokine staining
• Rare allergen-specific CD4 T-cell detection by activation-induced marker or tetramer approaches
• Basophil activation testing on autologous cells
• Regulatory T-cell function and suppression assays
• Oral immunotherapy and anti-IgE mechanism-of-action research
• Single-cell RNA sequencing and high-parameter immunophenotyping of the type 2 compartment
• Therapeutic screening and target validation in food allergy

Other Peanut Allergy Specimen Types

• Peanut Allergy Serum
• Peanut Allergy Plasma
• Peanut Allergy Whole Blood
• Peanut Allergy Leukopak
• Peanut Allergy Bulk Plasma
• Allergy Serum

Quality and Compliance

• IRB-approved collections and protocols
• 21 CFR Part 11 – compliant e-consent system
• HIPAA-compliant donor data protection

Ordering & Customization

Peanut Allergy PBMC samples are shipped on dry ice and available in custom aliquots. For pricing, current availability, or a quote on a stratified donor cohort, contact learnmore@sanguinebio.com; email us as well for international orders or regulatory documentation. To browse other disease states in this specimen type, visit our human PBMC page.