Type 1 Diabetes PBMC
Powered by BiozAbout:Type 1 Diabetes PBMC samples are collected from IRB-consented donors with clinically confirmed T1D and cryopreserved in CryoStor CS10. Ideal for autoreactive T cell detection, regulatory T cell assays, high-parameter immunophenotyping, TCR repertoire sequencing and single-cell profiling. Available stratified by islet autoantibody status, time since diagnosis and HLA genotype, with matched healthy controls.
About Type 1 Diabetes PBMC
Type 1 Diabetes PBMC for Autoimmune Diabetes Research
Type 1 Diabetes PBMC products are sourced from IRB-consented donors clinically diagnosed with type 1 diabetes (T1D), processed within 24 hours of collection and cryopreserved in CryoStor CS10. These peripheral blood mononuclear cells support autoreactive T cell work, immunophenotyping and functional assays.
T1D Autoimmunity and Why PBMC
Type 1 diabetes is an autoimmune disease in which insulin-producing pancreatic beta cells are selectively destroyed. This is mechanistically distinct from type 2 diabetes, which is metabolic rather than autoimmune.
- Autoreactive CD8+ and CD4+ T cells drive beta cell destruction, and islet-infiltrating specificities have peripheral counterparts detectable in blood
- Islet autoantibodies against GAD65, IA-2, insulin and ZnT8 are the clinical markers of the autoimmune process, and autoantibody number predicts progression in at-risk individuals
- HLA class II haplotypes, principally DR3-DQ2 and DR4-DQ8, confer the largest genetic risk, with additional contributions from loci including INS, PTPN22 and IL2RA
- Regulatory T cell function and the balance between effector and regulatory compartments is a long-standing focus and a target for immunotherapy
- Because pancreatic tissue is inaccessible in living donors, PBMC are the primary route to studying the human autoimmune response in T1D
- PBMC support tetramer-based antigen-specific detection, high-parameter flow cytometry, single-cell sequencing, Treg suppression assays and ex vivo stimulation
Donor Stratification Available
- Islet autoantibody status and specificity where available, including GAD65 and IA-2
- Time since diagnosis: new-onset versus long-standing disease
- HLA class II genotype where typed
- Glycaemic control by most recent HbA1c where available
- Insulin delivery method, including pump versus multiple daily injection
- Presence of diabetic complications or comorbid autoimmune disease such as celiac disease or autoimmune thyroid disease
- Matched healthy controls available
Product Features
- Research Use Only (RUO), cryopreserved PBMC
- Clinically confirmed T1D donors
- Available in cell counts ranging from 5 million to 2 billion
- Processed within 24 hours of collection
- Stored using CryoStor® CS10 freezing media
- IRB-approved protocols and electronic informed consent
- Screen LeukoLot™ available prior to bulk orders
De-identified Donor Data
- Verified T1D diagnosis
- Donor demographics: age, sex assigned at birth, race/ethnicity
- Self-reported allergies and infectious disease history
- Medication history including insulin regimen
- Additional T1D-specific data available upon request
Applications
- Autoreactive T cell identification, including tetramer-based antigen-specific detection
- High-parameter immunophenotyping of T cell, B cell and monocyte subsets
- Regulatory T cell frequency and suppression assays
- TCR repertoire sequencing and clonality analysis
- Single-cell and bulk transcriptomic profiling
- Immunotherapy and tolerance induction mechanism-of-action studies
- Genomic DNA extraction for HLA and risk-locus genotyping
- Biomarker discovery against matched healthy controls
Other Type 1 Diabetes Specimen Types
- Type 1 Diabetes Leukopak
- Type 1 Diabetes Plasma
- Type 1 Diabetes Serum
- Type 1 Diabetes Whole Blood
- Type 1 Diabetes CD3+ T Cells
- Type 1 Diabetes CD4+ T Cells
- Type 1 Diabetes CD8+ T Cells
Compliance and Quality Assurance
- IRB-approved collections and protocols
- 21 CFR Part 11 – compliant e-consent system
- HIPAA-compliant donor data protection
Ordering & Customization
Type 1 Diabetes PBMC samples are shipped on dry ice and available in custom aliquots. For pricing, availability, international orders or regulatory requirements, please contact learnmore@sanguinebio.com to confirm documentation and compliance needs. To browse PBMC from other disease states and check live stock status, visit our human PBMC page.
Donor Metadata
- Verified diagnosis and clinical history
- Demographic data: age, sex assigned at birth, race/ethnicity
- Medication and treatment background when available
Compliance and Quality Assurance
- IRB-approved collections and standardized procedures
- 21 CFR Part 11-compliant e-consent system
- HIPAA-compliant data management
Ordering & Customization
Human PBMC are available in customizable aliquot sizes. For international orders or documentation needs, please contact learnmore@sanguinebio.com to confirm requirements and availability. We also work with multiple ordering platforms and distributors, learn more here.
Protocols & Documentation
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PBMC Isolation from Whole Blood
Standard operating procedure for isolating peripheral blood mononuclear cells from whole blood using density-gradient separation.
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Immune Cell Isolation
Guidelines for isolating immune cell populations with validated enrichment steps, purity checkpoints, and documentation.
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Choosing the Right PBMC Configuration
Reference guide comparing PBMC formats, cryopreservation states, and study-fit recommendations for your application.
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Thawing Cryopreserved PBMC
Validated thawing protocol to recover viable cryopreserved PBMCs while minimizing activation and loss of function.
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Informed Consent Form (ICF)
Available upon request — confirms donor consent for research use and downstream commercialization.
PBMC
Where can I find your complete catalog?
You can find our full product catalog here.
How long can samples be stored?
Storage depends on sample type but often cryopreserved samples stored in liquid nitrogen can be stored for years.
What customization options are available?
Extensive customization is available. To discuss your project request a quote or email us at learnmore@sanguinebio.com.
Do you offer prospective collections?
Sanguine offers flexible prospective collection services tailored to fit your research. For more information and to request a quote, please see: our prospective biospecimen collection services page.
What is the turnaround time for custom collections?
Timelines depend on condition, sample type, and I/E criteria but we often start collection within 2 weeks of signed agreement.
Do you have samples in stock?
YES - we have in-stock inventory. You can also email us at learnmore@sanguinebio.com as we continually get new samples in our inventory.
What does unique donor mean?
Each sample from a different individual - Unique donor means each sample comes from a different person, ensuring biological diversity in your study.
Are samples collected under IRB-approved protocols?
YES - "IRB-approved collection protocols" and IRB approval documentation available upon request. Sanguine utilizes two internationally-recognized IRBs (Advarra and WCG IRB) for review and approval.
Frequently Asked Questions
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806 In Stock View InventoryNeed a custom PBMC cohort?
Our scientific team can scope prospective collections with donor-specific I/E criteria, typically starting within 2 weeks of agreement.