Type 1 Diabetes PBMC
Type 1 Diabetes PBMC samples are collected from IRB-consented donors with clinically confirmed T1D and cryopreserved in CryoStor CS10. Ideal for autoreactive T cell detection, regulatory T cell assays, high-parameter immunophenotyping, TCR repertoire sequencing and single-cell profiling. Available stratified by islet autoantibody status, time since diagnosis and HLA genotype, with matched healthy controls.
| Lot # | Condition | Cell Count (M) | Race/Ethnicity | Age | Gender | Country of Collection | Medications | Vials | Price | Action |
|---|---|---|---|---|---|---|---|---|---|---|
| 77367 | Diabetes Type I | 50M | Caucasian | 57 | Male | United States |
Skyrizi 150ml 4 times a year; Humalog insulin pump; levothyroxine 150mcg daily; atorvastatin 80 mg, losartan 25mg; zepbound glp 0.75 once a week
|
107 | $1,109.00 | |
| 77367 | Diabetes Type I | 10M | Caucasian | 57 | Male | United States |
Skyrizi 150ml 4 times a year; Humalog insulin pump; levothyroxine 150mcg daily; atorvastatin 80 mg, losartan 25mg; zepbound glp 0.75 once a week
|
12 | $530.00 | |
| 77153 | Diabetes Type I | 10M | Caucasian | 24 | Female | United States |
Tandem X2 Insulin Pump, Dexcom G7, Insulin: Novolog, Medical Marijuana
|
4 | $530.00 | |
| 77367 | Diabetes Type I | 50M | Caucasian | 57 | Male | United States |
Skyrizi 150ml 4 times a year; Humalog insulin pump; levothyroxine 150mcg daily; atorvastatin 80 mg, losartan 25mg; zepbound glp 0.75 once a week
|
1 | $1,109.00 | |
| 76212 | Diabetes Type I | 10M | Caucasian | 37 | Male | United States |
NOVOLOG 50 U/DAY
|
4 | $530.00 | |
| 76203 | Diabetes Type I | 10M | African American | 36 | Male | United States |
NOVOLOG/ 8u in morn/evening NOVLIN PEN 30U in morn and evening
|
2 | $530.00 | |
| 75225 | Diabetes Type I | 10M | Caucasian | 23 | Male | United States |
Insulin levetiracetam
|
6 | $530.00 | |
| 75202 | Diabetes Type I | 10M | Caucasian | 36 | Female | United States |
Humalog Insulin Pump Levothyroxine 0.175 mcg po QD Iron 65mg QD Multivitamin QD Calcium 600 QD D3 Qweek Biotin QD
|
8 | $530.00 | |
| 75055 | Diabetes Type I | 10M | Caucasian | 36 | Female | United States |
Lantus 24units in the morning Humalog PRN with meal
|
3 | $530.00 | |
| 75168 | Diabetes Type I | 10M | Caucasian | 30 | Female | United States |
Lantus and Humalog
|
8 | $530.00 |
Type 1 Diabetes PBMC for Autoimmune Diabetes Research
Type 1 Diabetes PBMC products are sourced from IRB-consented donors clinically diagnosed with type 1 diabetes (T1D), processed within 24 hours of collection and cryopreserved in CryoStor CS10. These peripheral blood mononuclear cells support autoreactive T cell work, immunophenotyping and functional assays.
T1D Autoimmunity and Why PBMC
Type 1 diabetes is an autoimmune disease in which insulin-producing pancreatic beta cells are selectively destroyed. This is mechanistically distinct from type 2 diabetes, which is metabolic rather than autoimmune.
- Autoreactive CD8+ and CD4+ T cells drive beta cell destruction, and islet-infiltrating specificities have peripheral counterparts detectable in blood
- Islet autoantibodies against GAD65, IA-2, insulin and ZnT8 are the clinical markers of the autoimmune process, and autoantibody number predicts progression in at-risk individuals
- HLA class II haplotypes, principally DR3-DQ2 and DR4-DQ8, confer the largest genetic risk, with additional contributions from loci including INS, PTPN22 and IL2RA
- Regulatory T cell function and the balance between effector and regulatory compartments is a long-standing focus and a target for immunotherapy
- Because pancreatic tissue is inaccessible in living donors, PBMC are the primary route to studying the human autoimmune response in T1D
- PBMC support tetramer-based antigen-specific detection, high-parameter flow cytometry, single-cell sequencing, Treg suppression assays and ex vivo stimulation
Donor Stratification Available
- Islet autoantibody status and specificity where available, including GAD65 and IA-2
- Time since diagnosis: new-onset versus long-standing disease
- HLA class II genotype where typed
- Glycaemic control by most recent HbA1c where available
- Insulin delivery method, including pump versus multiple daily injection
- Presence of diabetic complications or comorbid autoimmune disease such as celiac disease or autoimmune thyroid disease
- Matched healthy controls available
Product Features
- Research Use Only (RUO), cryopreserved PBMC
- Clinically confirmed T1D donors
- Available in cell counts ranging from 5 million to 2 billion
- Processed within 24 hours of collection
- Stored using CryoStor® CS10 freezing media
- IRB-approved protocols and electronic informed consent
- Screen LeukoLot™ available prior to bulk orders
De-identified Donor Data
- Verified T1D diagnosis
- Donor demographics: age, sex assigned at birth, race/ethnicity
- Self-reported allergies and infectious disease history
- Medication history including insulin regimen
- Additional T1D-specific data available upon request
Applications
- Autoreactive T cell identification, including tetramer-based antigen-specific detection
- High-parameter immunophenotyping of T cell, B cell and monocyte subsets
- Regulatory T cell frequency and suppression assays
- TCR repertoire sequencing and clonality analysis
- Single-cell and bulk transcriptomic profiling
- Immunotherapy and tolerance induction mechanism-of-action studies
- Genomic DNA extraction for HLA and risk-locus genotyping
- Biomarker discovery against matched healthy controls
Other Type 1 Diabetes Specimen Types
- Type 1 Diabetes Leukopak
- Type 1 Diabetes Plasma
- Type 1 Diabetes Serum
- Type 1 Diabetes Whole Blood
- Type 1 Diabetes CD3+ T Cells
- Type 1 Diabetes CD4+ T Cells
- Type 1 Diabetes CD8+ T Cells
Compliance and Quality Assurance
- IRB-approved collections and protocols
- 21 CFR Part 11 – compliant e-consent system
- HIPAA-compliant donor data protection
Ordering & Customization
Type 1 Diabetes PBMC samples are shipped on dry ice and available in custom aliquots. For pricing, availability, international orders or regulatory requirements, please contact learnmore@sanguinebio.com to confirm documentation and compliance needs. To browse PBMC from other disease states and check live stock status, visit our human PBMC page.
Protocols & Documentation
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Download
PBMC Isolation from Whole Blood
Standard operating procedure for isolating peripheral blood mononuclear cells from whole blood using density-gradient separation.
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Download
Immune Cell Isolation
Guidelines for isolating immune cell populations with validated enrichment steps, purity checkpoints, and documentation.
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Download
Choosing the Right PBMC Configuration
Reference guide comparing PBMC formats, cryopreservation states, and study-fit recommendations for your application.
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Download
Thawing Cryopreserved PBMC
Validated thawing protocol to recover viable cryopreserved PBMCs while minimizing activation and loss of function.
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Informed Consent Form (ICF)
Available upon request — confirms donor consent for research use and downstream commercialization.
PBMC
Frequently Asked Questions
1 Answer
YES – All collections are conducted under IRB-approved protocols and electronic informed consent. Sanguine utilizes Advarra and WCG IRB for oversight.
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YES – all our products are research use only (RUO).
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You can find our full product catalog here.
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Storage depends on sample type but often cryopreserved samples stored in liquid nitrogen can be stored for years.
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Extensive customization is available. To discuss your project request a quote or email us at learnmore@sanguinebio.com.
1 Answer
Sanguine offers flexible prospective collection services tailored to fit your research. For more information and to request a quote, please see: our prospective biospecimen collection services page.
1 Answer
Timelines depend on condition, sample type, and I/E criteria but we often start collection within 2 weeks of signed agreement.
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YES – we have in-stock inventory. You can also email us at learnmore@sanguinebio.com as we continually get new samples in our inventory.
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Each sample from a different individual – Unique donor means each sample comes from a different person, ensuring biological diversity in your study.
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YES – Unique donors can be specified and guaranteed based on your requirements.
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For information about sample quality, please see: Quality and Compliance
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YES – “IRB-approved collection protocols” and IRB approval documentation available upon request. Sanguine utilizes two internationally-recognized IRBs (Advarra and WCG IRB) for review and approval.
Ask a Question
Need a custom PBMC cohort?
Our scientific team can scope prospective collections with donor-specific I/E criteria, typically starting within 2 weeks of agreement.