About:Type 1 Diabetes PBMC samples are collected from consented donors with clinically confirmed T1D and cryopreserved in CryoStor CS10. Ideal for autoreactive T cell detection, regulatory T cell assays, high-parameter immunophenotyping, TCR repertoire sequencing and single-cell profiling. Available stratified by islet autoantibody status, time since diagnosis and HLA genotype, with matched healthy controls.
Insulin via pump Losartan 12.5mg (½ of 25mg tablet) QD – for kidneys (off-label) Xanax PRN – as needed Levothyroxine 137mcg QD, 6 days weekly Metoprolol Succinate ER 50mg QD – for heart Ezetimibe 10mg QD – for heart Imodium PRN – for bowels Cyclobenzaprine PRN – muscle relaxer for back Ozempic 2mg once weekly Simvastatin 20mg QD – cholesterol med Tremfya 100mg injection every 8 weeks – for psoriasis Cephalexin 500mg PRN – antibiotic for scratching/skin Baby Aspirin 81mg QD Vitamin D 2000 IU per day Pancrelipase (OTC) PRN – for pancreatic issues when eating fatty food Multivitamin Align (or similar) Probiotic QD
Insulin via pump Losartan 12.5mg (½ of 25mg tablet) QD – for kidneys (off-label) Xanax PRN – as needed Levothyroxine 137mcg QD, 6 days weekly Metoprolol Succinate ER 50mg QD – for heart Ezetimibe 10mg QD – for heart Imodium PRN – for bowels Cyclobenzaprine PRN – muscle relaxer for back Ozempic 2mg once weekly Simvastatin 20mg QD – cholesterol med Tremfya 100mg injection every 8 weeks – for psoriasis Cephalexin 500mg PRN – antibiotic for scratching/skin Baby Aspirin 81mg QD Vitamin D 2000 IU per day Pancrelipase (OTC) PRN – for pancreatic issues when eating fatty food Multivitamin Align (or similar) Probiotic QD
Type 1 Diabetes PBMC for Autoimmune Diabetes Research
Type 1 Diabetes PBMC products are sourced from IRB-consented donors clinically diagnosed with type 1 diabetes (T1D), processed within 24 hours of collection and cryopreserved in CryoStor CS10. These peripheral blood mononuclear cells support autoreactive T cell work, immunophenotyping and functional assays.
T1D Autoimmunity and Why PBMC
Type 1 diabetes is an autoimmune disease in which insulin-producing pancreatic beta cells are selectively destroyed. This is mechanistically distinct from type 2 diabetes, which is metabolic rather than autoimmune.
Autoreactive CD8+ and CD4+ T cells drive beta cell destruction, and islet-infiltrating specificities have peripheral counterparts detectable in blood
Islet autoantibodies against GAD65, IA-2, insulin and ZnT8 are the clinical markers of the autoimmune process, and autoantibody number predicts progression in at-risk individuals
HLA class II haplotypes, principally DR3-DQ2 and DR4-DQ8, confer the largest genetic risk, with additional contributions from loci including INS, PTPN22 and IL2RA
Regulatory T cell function and the balance between effector and regulatory compartments is a long-standing focus and a target for immunotherapy
Because pancreatic tissue is inaccessible in living donors, PBMC are the primary route to studying the human autoimmune response in T1D
PBMC support tetramer-based antigen-specific detection, high-parameter flow cytometry, single-cell sequencing, Treg suppression assays and ex vivo stimulation
Donor Stratification Available
Islet autoantibody status and specificity where available, including GAD65 and IA-2
Time since diagnosis: new-onset versus long-standing disease
HLA class II genotype where typed
Glycaemic control by most recent HbA1c where available
Insulin delivery method, including pump versus multiple daily injection
Presence of diabetic complications or comorbid autoimmune disease such as celiac disease or autoimmune thyroid disease
Matched healthy controls available
Product Features
Research Use Only (RUO), cryopreserved PBMC
Clinically confirmed T1D donors
Available in cell counts ranging from 5 million to 2 billion
Processed within 24 hours of collection
Stored using CryoStor® CS10 freezing media
IRB-approved protocols and electronic informed consent
Type 1 Diabetes PBMC samples are shipped on dry ice and available in custom aliquots. For pricing, availability, international orders or regulatory requirements, please contact learnmore@sanguinebio.com to confirm documentation and compliance needs. To browse PBMC from other disease states and check live stock status, visit our human PBMC page.
Applications
Immunology and translational research
Biomarker discovery and validation
Drug screening and assay development
Vaccine and infectious disease research
Product Features
Research Use Only (RUO), PBMC
Sourced from screened, consented donors
Processed under validated SOPs
QC tested for purity, viability, and sterility
Processed within 24 hours of collection
IRB-approved protocols with electronic informed consent
Donor Metadata
Verified diagnosis and clinical history
Demographic data: age, sex assigned at birth, race/ethnicity
Medication and treatment background when available
Compliance and Quality Assurance
IRB-approved collections and standardized procedures
21 CFR Part 11-compliant e-consent system
HIPAA-compliant data management
Ordering & Customization
Human PBMC are available in customizable aliquot sizes. For international orders or documentation needs, please contact learnmore@sanguinebio.com to confirm requirements and availability. We also work with multiple ordering platforms and distributors, learn more here.
Protocols & Documentation
PBMC Isolation from Whole Blood
Standard operating procedure for isolating peripheral blood mononuclear cells from whole blood using density-gradient separation.
What is the turnaround time for custom collections?
Timelines depend on condition, sample type, and I/E criteria but we often start collection within 2 weeks of signed agreement.
Do you have samples in stock?
YES - we have in-stock inventory. You can also email us at learnmore@sanguinebio.com as we continually get new samples in our inventory.
What does unique donor mean?
Each sample from a different individual - Unique donor means each sample comes from a different person, ensuring biological diversity in your study.
Are samples collected under IRB-approved protocols?
YES - "IRB-approved collection protocols" and IRB approval documentation available upon request. Sanguine utilizes two internationally-recognized IRBs (Advarra and WCG IRB) for review and approval.
Frequently Asked Questions
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