Human Immunodeficiency Virus HIV Leukopak

High-Yield LeukoCore HIV Leukopaks for Immunology Research

LeukoCore Human Immunodeficiency Virus (HIV) Leukopak biospecimens are collected from donors clinically diagnosed with HIV through IRB-approved apheresis procedures. Each leukopak contains large quantities of peripheral blood mononuclear cells (PBMCs), making it ideal for immune profiling, cytokine analysis, and drug development research related to HIV.

Why Apheresis Volume Matters for HIV Reservoir Work

The latent HIV reservoir is vanishingly rare — typically on the order of one infected cell per million resting CD4+ T cells in donors on suppressive therapy. Assays that quantify it, including quantitative viral outgrowth and intact proviral DNA assays, therefore need very large cell inputs to return a reliable measurement. A standard blood draw usually cannot supply enough. A leukopak can, which is why apheresis material is effectively a prerequisite for reservoir and cure research rather than a convenience.

Donor Stratification Available

  • Viral load — HIV-1 RNA copies/mL where tested
  • CD4+ count and CD4/CD8 ratio where recorded
  • Treatment status — treatment-naive, or on suppressive ART with duration
  • Elite controllers and long-term non-progressors — available as custom collections
  • HLA type — including protective alleles such as HLA-B*57:01
  • Co-infections — hepatitis B, hepatitis C, CMV serostatus

Product Features

  • Research Use Only (RUO) human leukopaks
  • Clinically confirmed HIV donors
  • High cell yield for large-scale studies
  • Processed and shipped within 24 hours of collection
  • Fresh or cryopreserved options available
  • Half and full leukopak formats
  • IRB-approved and ethically sourced
  • Infectious disease testing
  • HLA-A typing
  • Biosafety-compliant handling and shipping
  • Screen donors with LeukoLot™

De-identified Donor Data

  • Verified HIV diagnosis from healthcare providers
  • Donor demographics: age, sex assigned at birth, race/ethnicity
  • Self-reported medication history and allergies
  • Relevant clinical data

Applications

  • HIV latent reservoir quantification (QVOA, IPDA)
  • Latency reversal agent screening
  • Immune cell characterization in HIV infection
  • CD4/CD8 immunophenotyping and exhaustion profiling
  • Therapeutic development and screening, including cure strategies
  • Cell-based assays and cytokine profiling
  • Multi-omics studies including single-cell sequencing

Compliance and Quality Assurance

  • Collected under IRB oversight with donor e-consent (21 CFR Part 11 compliant)
  • HIPAA-compliant handling of donor information
  • Rigorous donor screening and documentation

Other HIV Specimen Types

Matched material is available as HIV PBMC, plasma, serum, whole blood, CD3+ T cells, and bulk apheresis plasma.

Ordering & Customization

HIV Leukopak samples are available fresh or cryopreserved. Need a different condition, or want to check current pricing and availability across our full leukopak inventory? Visit our human leukopak page to browse other disease-state options and live stock status. For international shipping or regulatory documentation, please contact learnmore@sanguinebio.com to confirm regional compliance and shipping availability.

Protocols & Documentation

  • Leukopak Cryopreservation

    Standard operating procedure for cryopreserving leukopak units with controlled freezing and post-thaw viability targets.

    Download
  • Leukopak Thawing

    Validated thawing workflow for cryopreserved leukopaks to maximize recovery while maintaining sterility and chain of custody.

    Download
  • Choosing the Right PBMC Configuration

    Reference guide comparing PBMC formats, cryopreservation states, and study-fit recommendations for your application.

    Download
  • Informed Consent Form (ICF)

    Available upon request — confirms donor consent for research use and downstream commercialization.

Leukopak

Frequently Asked Questions

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Are samples IRB approved?

1 Answer

YES – All collections are conducted under IRB-approved protocols and electronic informed consent. Sanguine utilizes Advarra and WCG IRB for oversight.

YES – all our products are research use only (RUO).

Storage depends on sample type but often cryopreserved samples stored in liquid nitrogen can be stored for years.

Extensive customization is available. To discuss your project request a quote or email us at learnmore@sanguinebio.com.

Sanguine offers flexible prospective collection services tailored to fit your research. For more information and to request a quote, please see: our prospective biospecimen collection services page.

Timelines depend on condition, sample type, and I/E criteria but we often start collection within 2 weeks of signed agreement.

YES – we have in-stock inventory. You can also email us at learnmore@sanguinebio.com as we continually get new samples in our inventory.

Each sample from a different individual – Unique donor means each sample comes from a different person, ensuring biological diversity in your study.

YES – Unique donors can be specified and guaranteed based on your requirements.

For information about sample quality, please see: Quality and Compliance

YES – “IRB-approved collection protocols” and IRB approval documentation available upon request. Sanguine utilizes two internationally-recognized IRBs (Advarra and WCG IRB) for review and approval.

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Need a custom Leukopak cohort?

Our scientific team can scope prospective collections with donor-specific I/E criteria, typically starting within 2 weeks of agreement.

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