Rheumatoid Arthritis PBMC
Powered by BiozAbout:Rheumatoid Arthritis PBMC samples are collected from consented donors with clinically confirmed RA and cryopreserved in CryoStor CS10. Ideal for immunophenotyping T cell, B cell and monocyte subsets, Th17 and Treg balance studies, cytokine signalling assays and biologic mechanism-of-action work. Available stratified by ACPA status, disease activity and treatment class, with matched healthy controls.
About Rheumatoid Arthritis PBMC
Rheumatoid Arthritis PBMC for Autoimmune and Inflammation Research
Rheumatoid Arthritis Human PBMC products are sourced from IRB-consented donors clinically diagnosed with rheumatoid arthritis (RA), processed within 24 hours of collection and cryopreserved in CryoStor CS10. These peripheral blood mononuclear cells support T cell, B cell and monocyte studies relevant to autoimmune activity and therapeutic target validation.
RA Autoimmunity and Why PBMC
Rheumatoid arthritis is a systemic autoimmune disease that targets synovial joints, producing chronic synovitis and, if uncontrolled, progressive cartilage and bone erosion.
- Anti-citrullinated protein antibodies (ACPA) and rheumatoid factor define the seropositive subset, and ACPA can precede clinical arthritis by years
- The shared epitope alleles of HLA-DRB1 are the dominant genetic risk factor, and PTPN22 variants contribute additional risk
- CD4+ T cell help, including Th17 responses, drives B cell activation and autoantibody class switching; B cell depletion is an effective clinical strategy, which underlines the B cell contribution
- Monocytes and macrophages are the principal sources of TNF-alpha and IL-6, the cytokines targeted by the most widely used biologic therapies
- Peripheral monocyte, T cell and B cell phenotypes shift with disease activity and with treatment, making viable PBMC the appropriate specimen for mechanism and pharmacodynamic work
- PBMC support high-parameter flow cytometry, single-cell sequencing, JAK-STAT and cytokine signalling assays, and ex vivo drug response testing
Donor Stratification Available
- Seropositive versus seronegative disease by ACPA and rheumatoid factor status
- Disease activity: active disease versus low disease activity or remission
- Treatment status: treatment-naive, on conventional synthetic DMARDs such as methotrexate, on TNF inhibitors, on IL-6 receptor blockade, or on JAK inhibitors
- Disease duration: early versus established RA
- Presence of erosive disease or extra-articular manifestations
- Matched healthy controls available
Product Features
- Research Use Only (RUO), cryopreserved PBMCs
- Clinically confirmed RA donors
- Available in cell counts ranging from 5 million to 2 billion
- Processed within 24 hours of collection
- Stored using CryoStor® CS10 freezing media
- IRB-approved protocols and electronic informed consent
- Screen LeukoLot™ available prior to bulk orders
De-identified Donor Data
- Verified RA diagnosis
- Donor demographics: age, sex assigned at birth, race/ethnicity
- Self-reported allergies and infectious disease history
- Medication history including DMARD and biologic therapy
- Additional RA-specific data available upon request
Applications
- High-parameter immunophenotyping of T cell, B cell and monocyte subsets
- Th17 and regulatory T cell balance studies
- Ex vivo TNF-alpha and IL-6 production and cytokine signalling assays
- JAK inhibitor and biologic mechanism-of-action research
- Single-cell and bulk transcriptomic profiling
- B cell depletion and autoantibody-producing cell studies
- Target identification and validation for autoimmune inflammation
- Treatment-response biomarker discovery against matched controls
Other Rheumatoid Arthritis Specimen Types
- Rheumatoid Arthritis Plasma
- Rheumatoid Arthritis Serum
- Rheumatoid Arthritis Whole Blood
- Rheumatoid Arthritis Synovial Fluid
- Rheumatoid Arthritis CD3+ T Cells
- Rheumatoid Arthritis CD19+ B Cells
- Rheumatoid Arthritis Bulk Plasma
Compliance and Quality Assurance
- IRB-approved collections and protocols
- 21 CFR Part 11 – compliant e-consent system
- HIPAA-compliant donor data protection
Ordering & Customization
Rheumatoid Arthritis PBMC samples are shipped on dry ice and available in custom aliquots. For pricing, availability, international orders or regulatory requirements, please contact learnmore@sanguinebio.com to confirm documentation and compliance needs. To browse PBMC from other disease states and check live stock status, visit our human PBMC page.
Applications
- Immunology and translational research
- Biomarker discovery and validation
- Drug screening and assay development
- Vaccine and infectious disease research
Product Features
- Research Use Only (RUO), PBMC
- Sourced from screened, consented donors
- Processed under validated SOPs
- QC tested for purity, viability, and sterility
- Processed within 24 hours of collection
- IRB-approved protocols with electronic informed consent
Donor Metadata
- Verified diagnosis and clinical history
- Demographic data: age, sex assigned at birth, race/ethnicity
- Medication and treatment background when available
Compliance and Quality Assurance
- IRB-approved collections and standardized procedures
- 21 CFR Part 11-compliant e-consent system
- HIPAA-compliant data management
Ordering & Customization
Human PBMC are available in customizable aliquot sizes. For international orders or documentation needs, please contact learnmore@sanguinebio.com to confirm requirements and availability. We also work with multiple ordering platforms and distributors, learn more here.
Protocols & Documentation
-
Download
PBMC Isolation from Whole Blood
Standard operating procedure for isolating peripheral blood mononuclear cells from whole blood using density-gradient separation.
-
Download
Immune Cell Isolation
Guidelines for isolating immune cell populations with validated enrichment steps, purity checkpoints, and documentation.
-
Download
Choosing the Right PBMC Configuration
Reference guide comparing PBMC formats, cryopreservation states, and study-fit recommendations for your application.
-
Download
Thawing Cryopreserved PBMC
Validated thawing protocol to recover viable cryopreserved PBMCs while minimizing activation and loss of function.
-
Informed Consent Form (ICF)
Available upon request — confirms donor consent for research use and downstream commercialization.
PBMC
Where can I find your complete catalog?
You can find our full product catalog here.
How long can samples be stored?
Storage depends on sample type but often cryopreserved samples stored in liquid nitrogen can be stored for years.
What customization options are available?
Extensive customization is available. To discuss your project request a quote or email us at learnmore@sanguinebio.com.
Do you offer prospective collections?
Sanguine offers flexible prospective collection services tailored to fit your research. For more information and to request a quote, please see: our prospective biospecimen collection services page.
What is the turnaround time for custom collections?
Timelines depend on condition, sample type, and I/E criteria but we often start collection within 2 weeks of signed agreement.
Do you have samples in stock?
YES - we have in-stock inventory. You can also email us at learnmore@sanguinebio.com as we continually get new samples in our inventory.
What does unique donor mean?
Each sample from a different individual - Unique donor means each sample comes from a different person, ensuring biological diversity in your study.
Are samples collected under IRB-approved protocols?
YES - "IRB-approved collection protocols" and IRB approval documentation available upon request. Sanguine utilizes two internationally-recognized IRBs (Advarra and WCG IRB) for review and approval.
Frequently Asked Questions
Ask a Question
Pair with a Healthy Control
Match your Rheumatoid Arthritis PBMC with normal donor samples for comparison
Healthy Donor PBMCs
705 In Stock View InventoryNeed a custom PBMC cohort?
Our scientific team can scope prospective collections with donor-specific I/E criteria, typically starting within 2 weeks of agreement.