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Techniques in Cytology – Cytospin: Cytospin Cell Resuspension Solution
In this blog series opening article, I discussed how cytospins…
Techniques in Cytology – Cytospin: Distinguishing Benign Cells from Malignant Cells
Cytology is a key component in diagnosis and screening of…
Using Mass Spectrometry for Mass T cell Epitope Discovery
Time of Flight Mass Cytometry (CyTOF) is a relatively new…
Whole Blood Phospho-flow: Direct Ex Vivo Measurement of Signaling in PBMC
I previously discussed phospho-flow cytometry, a method to study intracellular…
Positive Selection vs Negative Selection for Cell Isolation
In a previous post, I covered the current options for…
Optimizing Assays to Find Rare Antigen-Specific T cells in Cryopreserved PBMCs
Immunomonitoring of T cell based immune responses spans a wide…
Highlight: How TNF knocks out Tregs!
A healthy and functional immune system requires a delicate balance…
Considerations for measuring cytokine levels in serum or plasma
Changes in circulating cytokine and chemokine levels have been associated…
Using Application Settings to Standardize Flow Cytometry Results Across Experiments and Instruments
While many fluorescence-based flow cytometry assays can be run without…