Rheumatoid Arthritis Leukopak

About Rheumatoid Arthritis Leukopak

LeukoCore Rheumatoid Arthritis Leukopak for Autoimmune and Immunology Research

LeukoCore Rheumatoid Arthritis Leukopak biospecimens are collected from IRB-consented donors clinically diagnosed with rheumatoid arthritis (RA) through IRB-approved apheresis procedures. Rheumatoid arthritis is a genuinely autoimmune disease: loss of tolerance to citrullinated self-proteins gives rise to anti-citrullinated protein antibodies (ACPA) and rheumatoid factor, and the resulting synovial inflammation destroys cartilage and bone. Each leukopak contains large quantities of peripheral blood mononuclear cells, supporting cell-intensive work in autoimmunity, B cell biology and bone destruction.

Why a Leukopak for Rheumatoid Arthritis Research

• ACPA and rheumatoid factor are produced by autoreactive B cells and plasmablasts that circulate at low frequency. Isolating and characterising antigen-specific B cells requires the cell numbers only apheresis can deliver.
• The strongest genetic risk in RA lies in the HLA-DRB1 shared epitope, which shapes presentation of citrullinated peptides to CD4+ T cells. Leukopaks provide enough CD4 T cells for antigen-specific and tetramer-based work in HLA-typed donors.
• Peripheral helper T cells and other B cell-helping T populations described in RA are rare subsets; recovering them in usable numbers for functional assays is a practical argument for leukopak rather than venipuncture collection.
• Bone erosion in RA is mediated by RANKL-driven osteoclasts differentiated from the monocyte lineage. A single leukopak supplies enough monocytes to generate osteoclasts in vitro and study erosive potential in patient-derived cells.
• RA is the disease in which TNF, IL-6 receptor, JAK, CD20 and CD28-costimulation blockade are all clinically established. Leukopak-scale material allows several mechanism-of-action readouts to be run in parallel on one donor rather than split across cohorts.
• High cell yields support engineered cell approaches — including regulatory T cell expansion and CAR-based tolerance strategies — that are increasingly explored in autoimmune disease.

Donor Stratification Available

• Serology: ACPA-positive, rheumatoid factor-positive, dual-positive, or seronegative RA
• Disease activity by DAS28 or CDAI where recorded
• Treatment status: biologic-naive, on methotrexate or other conventional synthetic DMARD, on TNF inhibitor, on IL-6 receptor inhibitor, on JAK inhibitor, on rituximab or abatacept, or in washout
• Disease duration: early versus established RA
• Erosive versus non-erosive disease where radiographic status is reported
• Extra-articular involvement and comorbidity, including interstitial lung disease
• Matched healthy controls available

Product Features

• Research Use Only (RUO) human leukopaks
• Clinically confirmed RA donors
• High cell yield for large-scale studies
• Processed and shipped within 24 hours of collection
• Fresh or cryopreserved options available
• IRB-approved and ethically sourced
• Infectious disease testing
• HLA-A typing
• Screen donors with LeukoLot™

De-identified Donor Data

• Verified RA diagnosis from healthcare providers
• Donor demographics: age, sex assigned at birth, race/ethnicity
• Self-reported medication history and allergies
• Serology and disease activity where available
• Relevant clinical data

Applications

• Autoreactive and antigen-specific B cell isolation and repertoire analysis
• CD4+ T cell subset isolation, including B cell-helping and peripheral helper populations
• Monocyte-derived osteoclast differentiation and bone resorption assays
• Mechanism-of-action studies for TNF, IL-6R, JAK, CD20 and costimulation-directed therapeutics
• Regulatory T cell expansion and engineered cell therapy development
• Cytokine profiling and cell-based functional assays
• Multi-omics studies including single-cell RNA sequencing and immune repertoire sequencing
• Immunophenotyping of seropositive versus seronegative RA

Other Rheumatoid Arthritis Specimen Types

• Rheumatoid Arthritis PBMC
• Rheumatoid Arthritis Serum
• Rheumatoid Arthritis Plasma
• Rheumatoid Arthritis Whole Blood
• Rheumatoid Arthritis Synovial Fluid
• Rheumatoid Arthritis Bulk Plasma

Compliance and Quality Assurance

• Collected under IRB oversight with donor e-consent (21 CFR Part 11 compliant)
• HIPAA-compliant handling of donor information
• Rigorous donor screening and documentation

Ordering & Customization

Rheumatoid Arthritis Leukopak is available fresh or cryopreserved. For pricing, current availability, or to discuss a serology-stratified cohort, contact learnmore@sanguinebio.com. Need a different condition, or want to check current availability across our full leukopak inventory? Visit our human leukopak page to browse other disease-state options and live stock status. For international shipping or regulatory documentation, please contact us to confirm regional compliance and shipping availability.