Rheumatoid Arthritis Whole Blood

About Rheumatoid Arthritis Whole Blood

Rheumatoid Arthritis Whole Blood for Autoimmune and Rheumatology Research

Rheumatoid arthritis (RA) is a systemic autoimmune disease in which anti-citrullinated protein antibodies (ACPA, measured clinically as anti-CCP) and rheumatoid factor drive persistent synovial inflammation, pannus formation and progressive joint erosion. Sanguine collects fresh whole blood from IRB-consented donors with a clinically confirmed RA diagnosis for rheumatology, autoimmunity and immunology research.

Why Whole Blood for Rheumatoid Arthritis Research

• Whole blood is the only routine peripheral specimen that retains every leukocyte lineage. Neutrophils and low-density granulocytes are lost during density-gradient PBMC isolation, yet neutrophils are central to RA: they are the dominant cell in synovial fluid and they externalise citrullinated autoantigen through extracellular trap formation.
• Because whole blood preserves the native leukocyte composition, it supports unbiased transcriptomic profiling in which granulocyte and platelet signatures are preserved rather than removed by upstream cell separation.
• ACPA and rheumatoid factor can be detected years before clinical synovitis, and the ACPA-positive and ACPA-negative subsets differ in genetics, including the HLA-DRB1 shared epitope association. Whole blood yields matched genomic DNA for HLA and risk-allele genotyping alongside the same donor’s serology.
• Whole blood supports ex vivo stimulation in a physiological matrix, so TNF, IL-6 and JAK/STAT pathway responses can be measured with the plasma proteins, complement and erythrocytes still present.
• RA is treated across mechanistically distinct classes including methotrexate, TNF inhibitors, IL-6 receptor blockade, T-cell costimulation blockade, B-cell depletion and JAK inhibitors. Whole blood from donors on each class enables pharmacodynamic and target-engagement assay development.
• Fresh whole blood also allows same-day functional assays such as flow cytometry immunophenotyping, whole-blood cytokine release, platelet studies and viability-sensitive workflows that cryopreserved fractions cannot support.

Donor Stratification Available

• Serostatus: anti-CCP/ACPA positive or negative, and rheumatoid factor positive or negative
• Disease activity where documented, including DAS28, CDAI, ESR and CRP, and active disease versus remission
• Disease duration and erosive versus non-erosive radiographic status
• Treatment status: treatment-naive, conventional synthetic DMARD, TNF inhibitor, IL-6 receptor inhibitor, T-cell costimulation blockade, B-cell depletion, or JAK inhibitor
• Extra-articular and comorbid features where documented, including interstitial lung disease, nodules and secondary Sjogren’s syndrome
• Matched healthy controls available, and other inflammatory arthritis donors such as psoriatic arthritis for comparison

Product Features

• Fresh whole blood from clinically diagnosed RA donors
• Custom anticoagulants and collection tubes available, including EDTA, sodium heparin, ACD and RNA-stabilising tubes
• Same-day collection and shipment
• Research Use Only (RUO)
• Draw volumes specified per collection and reported per lot

De-identified Donor Data

• Physician-confirmed diagnosis, including anti-CCP and RF status where available
• Demographics: age, sex assigned at birth, ethnicity
• Relevant medical and treatment history
• Additional RA-specific clinical detail available on request

Applications

• Anti-CCP/ACPA and rheumatoid factor autoantibody research
• Whole-blood immunophenotyping including granulocyte and low-density neutrophil populations
• Whole-blood transcriptomics, gene expression signatures and epigenetic profiling
• Ex vivo stimulation and cytokine release assays in a physiological matrix
• Pharmacodynamic and target-engagement assays for DMARD, biologic and JAK inhibitor programmes
• Genomic DNA extraction for HLA-DRB1 and risk-allele genotyping
• Neutrophil extracellular trap and citrullination research
• Source material for downstream PBMC, granulocyte, plasma and serum preparation

Other Rheumatoid Arthritis Specimen Types

• Rheumatoid Arthritis Serum
• Rheumatoid Arthritis Plasma
• Rheumatoid Arthritis PBMC
• Rheumatoid Arthritis Leukopak
• Rheumatoid Arthritis Bulk Plasma
• Rheumatoid Arthritis Synovial Fluid
• Rheumatoid Arthritis CD19+ B Cells
• Rheumatoid Arthritis CD4+ T Cells

Compliance and Quality Assurance

• IRB-approved protocols and electronic informed consent
• HIPAA-compliant donor data handling
• Documentation support available for regulatory review

Ordering & Customization

RA whole blood is available in standard and custom volumes. For pricing, current availability, or a quote on a stratified donor cohort, email learnmore@sanguinebio.com. Email us as well for international orders or documentation requirements. To browse other disease states in this specimen type, visit our human whole blood page.